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https://hdl.handle.net/1822/72255
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Campo DC | Valor | Idioma |
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dc.contributor.author | Purwidyantri, Agnes | por |
dc.contributor.author | Domingues, Telma | por |
dc.contributor.author | Borme, Jérôme | por |
dc.contributor.author | Guerreiro, Joana Rafaela | por |
dc.contributor.author | Ipatov, Andrey | por |
dc.contributor.author | Abreu, Catarina M. | por |
dc.contributor.author | Martins, Marco | por |
dc.contributor.author | Alpuim, P. | por |
dc.contributor.author | Prado, Marta | por |
dc.date.accessioned | 2021-04-22T22:35:12Z | - |
dc.date.available | 2021-04-22T22:35:12Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Purwidyantri, A.; Domingues, T.; Borme, J.; Guerreiro, J.R.; Ipatov, A.; Abreu, C.M.; Martins, M.; Alpuim, P.; Prado, M. Influence of the Electrolyte Salt Concentration on DNA Detection with Graphene Transistors. Biosensors 2021, 11, 24. https://doi.org/10.3390/bios11010024 | por |
dc.identifier.issn | 2079-6374 | - |
dc.identifier.uri | https://hdl.handle.net/1822/72255 | - |
dc.description.abstract | Liquid-gated Graphene Field-Effect Transistors (GFET) are ultrasensitive bio-detection platforms carrying out the graphene’s exceptional intrinsic functionalities. Buffer and dilution factor are prevalent strategies towards the optimum performance of the GFETs. However, beyond the Debye length (λD), the role of the graphene-electrolytes’ ionic species interactions on the DNA behavior at the nanoscale interface is complicated. We studied the characteristics of the GFETs under different ionic strength, pH, and electrolyte type, e.g., phosphate buffer (PB), and phosphate buffer saline (PBS), in an automatic portable built-in system. The electrostatic gating and charge transfer phenomena were inferred from the field-effect measurements of the Dirac point position in single-layer graphene (SLG) transistors transfer curves. Results denote that <i>λ<sub>D</sub></i> is not the main factor governing the effective nanoscale screening environment. We observed that the longer <i>λ<sub>D</sub></i> was not the determining characteristic for sensitivity increment and limit of detection (LoD) as demonstrated by different types and ionic strengths of measuring buffers. In the DNA hybridization study, our findings show the role of the additional salts present in PBS, as compared to PB, in increasing graphene electron mobility, electrostatic shielding, intermolecular forces and DNA adsorption kinetics leading to an improved sensitivity. | por |
dc.description.sponsorship | This research is supported by PORTGRAPHE-Control of Port and DouroWines authenticity using graphene DNA sensors project co-funded by FCT (PTDC/BIA-MOL/31069/2017) and the ERDF through COMPETE2020 (POCI-01-0145-FEDER-031069). | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute | por |
dc.relation | PTDC/BIA-MOL/31069/2017 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | liquid gate | por |
dc.subject | graphene | por |
dc.subject | Debye length | por |
dc.subject | phosphate buffer (PB) | por |
dc.subject | phosphate buffer saline (PBS) | por |
dc.subject | salts | por |
dc.subject | DNA | por |
dc.title | Influence of the electrolyte salt concentration on DNA detection with graphene transistors | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/2079-6374/11/1/24 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 11 | por |
dc.date.updated | 2021-01-22T15:46:58Z | - |
dc.identifier.doi | 10.3390/bios11010024 | por |
dc.identifier.pmid | 33477344 | por |
dc.subject.fos | Ciências Naturais::Ciências Físicas | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biosensors | por |
oaire.version | VoR | por |
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biosensors-11-00024.pdf | 3,02 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons