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https://hdl.handle.net/1822/72046
Título: | Ligninolytic enzymes production during polycyclic aromatic hydrocarbons degradation: effect of soil pH, soil amendments and fungal co-cultivation |
Autor(es): | Vipotnik, Ziva Michelin, Michele Tavares, T. |
Palavras-chave: | PAHs Soil remediation Laccase Manganese peroxidase Lignin peroxidase Fungi |
Data: | 2021 |
Editora: | Springer Nature |
Revista: | Biodegradation |
Citação: | Vipotnik, Z.; Michelin, Michele; Tavares, Teresa, Ligninolytic enzymes production during polycyclic aromatic hydrocarbons degradation: effect of soil pH, soil amendments and fungal co-cultivation. Biodegradation, 32, 193-215, 2021 |
Resumo(s): | Soil microorganisms play an important role in the degradation of PAHs and use various metabolic pathways for this process. The effect of soil pH, different soil amendments and the co-cultivation of fungi on the degradation of PAHs in soil and on the activity of ligninolytic enzymes was evaluated. For that purpose, three fungi were studied: Trichoderma viride, Penicillium chrysogenum and Agrocybe aegerita. Biodegradation assays with a mixture of 200 ppm PAHs (fluorene, pyrene, chrysene, and benzo[a]pyrene---50 ppm each) were set up at room temperature for 8 weeks. The maximum laccase activity by solid state fermentation---SSF (7.43 U/g) was obtained by A. aegerita on kiwi peels with 2 weeks and the highest manganese peroxidase activity (7.21 U/g) was reached in 4 weeks, both at pH 7. Fluorene, pyrene, and benzo[a]pyrene achieved higher degradation rates in soil at pH 5, while chrysene was more degradable at pH 7. About 85--90\% of the PAHs were degraded by fungal remediation. The highest degradation of fluorene was achieved by co-cultivation of A. aegerita and P. chrysogenum, remaining 14\% undegradable. Around 13\% of pyrene stay undegradable by A. aegerita and T. viride and by A. aegerita and P. chrysogenum, both systems supported in kiwi peels, while 11\% of chrysene remained in soil by the co-cultivation of these fungi, supported by peanut shells. Regarding benzo[a]pyrene, 13\\% remained in soil after treatment with A. aegerita. Despite the increase in degradation of some PAHs with co-cultivation, higher enzyme production during degradation was observed when fungi were cultivated alone. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/72046 |
DOI: | 10.1007/s10532-021-09933-2 |
ISSN: | 0923-9820 |
Versão da editora: | http://www.springerlink.com/content/0923-9820/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54309_1.pdf | 618,78 kB | Adobe PDF | Ver/Abrir |