Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/71550
Título: | Optical biosensor for catechol determination based on laccase‐immobilized anionic polyamide 6 microparticles |
Autor(es): | Cano‐Raya, Clara Dencheva, Nadya Vasileva Braz, Joana F. Malfois, Marc Denchev, Z. |
Palavras-chave: | microscopy optical properties ring-opening polymerization sensors and actuators X-ray |
Data: | 2020 |
Editora: | John Wiley & Sons |
Revista: | Journal of Applied Polymer Science |
Citação: | Cano‐Raya, C, Dencheva, NV, Braz, JF, Malfois, M, Denchev, ZZ. Optical biosensor for catechol determination based on laccase‐immobilized anionic polyamide 6 microparticles. J Appl Polym Sci. 2020; 137:e49131. https://doi.org/10.1002/app.49131 |
Resumo(s): | This work presents the preparation, optimization, and testing of an enzymebased optical biosensor for catechol determination. The sensing area is attached to a glass support and contains: anionic polyamide 6 (PA6) porous microparticles supporting laccase from Trametes Versicolor, embedded in a Pebax® MH1657 polymer binder that contains the optical indicator dye 3-methyl-2-benzothiazolinone hydrazone (MBTH), responsible for the optical transduction. The catechol analyte, after its enzymatic oxidation, forms o-benzoquinone that can be detected by oxidative coupling with MBTH giving rise to a colored product. The latter can be quantified easuring the UV/VIS absorbance at 500 nm. The PA6 microparticles performed as useful laccase carriers reaching high immobilization yields of up to 99.8% and preserving the enzyme catalytic activity. This permitted the reparation of a new biosensor presenting a detection limit of 11 μM and responding linearly to up to 118 μM of catechol. Biosensor applicability was tested in spiked natural water samples from river and spring. The recovery rates observed were in the range of 97–108% that proves the good accuracy of the proposed biosensor. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/71550 |
DOI: | 10.1002/app.49131 |
ISSN: | 0021-8995 |
e-ISSN: | 1097-4628 |
Versão da editora: | https://onlinelibrary.wiley.com/doi/full/10.1002/app.49131 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | IPC - Artigos em revistas científicas internacionais com arbitragem |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Cano-Raya et al - JAPPS 2020.pdf | Versão Final da editora | 4,84 MB | Adobe PDF | Ver/Abrir |