Analysis of the effects of hyperbaric gases on S. cerevisiae cell cycle through a morphological approach

Abstract

The effects of hyperbaric gases on the cell cycle of Saccharomyces cerevisiae were studied in batch cultures under pressures between 0.1 and 0.6 MPa and different gas compositions (air, oxygen, nitrogen or carbon dioxide). Classification of S. cerevisiae cells based on their morphology stages was obtained using an automatic image analysis procedure. Information on the distribution of different sub-populations along the cell cycle is reported. A structured morphological model was developed and used to describe the measured data. The results herein reported demonstrate that the bud separation phase is the limiting step in cell duplication. Additionally, the influence of the environmental conditions, specially the oxygen partial pressure, on the START event is reported. Under anaerobic conditions, no significant influence of hyperbaric gases on the cell cycle was verified.