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https://hdl.handle.net/1822/67938
Título: | Macrophages and myeloid dendritic cells, but not plasmacytoid dendritic cells, produce IL-10 in response to MyD88- and TRIF-dependent TLR signals, and TLR-independent signals |
Autor(es): | Boonstra, André Rajsbaum, Ricardo Holman, Mary Marques, Rute Asselin-Paturel, Carine Pereira, João Pedro Bates, Elizabeth E. M. Akira, Shizuo Vieira, Paulo Liu, Yong-Jun Trinchieri, Giorgio O'Garra, Anne |
Palavras-chave: | Adaptor Proteins, Vesicular Transport Animals Bone Marrow Cells CD8 Antigens CpG Islands Dendritic Cells Interleukin-10 Interleukin-12 Macrophages Mice Myeloid Cells Myeloid Differentiation Factor 88 Spleen Toll-Like Receptors |
Data: | 2006 |
Editora: | American Association of Immunologists |
Revista: | The Journal of Immunology |
Resumo(s): | We have previously reported that mouse plasmacytoid dendritic cells (DC) produce high levels of IL-12p70, whereas bone marrow-derived myeloid DC and splenic DC produce substantially lower levels of this cytokine when activated with the TLR-9 ligand CpG. We now show that in response to CpG stimulation, high levels of IL-10 are secreted by macrophages, intermediate levels by myeloid DC, but no detectable IL-10 is secreted by plasmacytoid DC. MyD88-dependent TLR signals (TLR4, 7, 9 ligation), Toll/IL-1 receptor domain-containing adaptor-dependent TLR signals (TLR3, 4 ligation) as well as non-TLR signals (CD40 ligation) induced macrophages and myeloid DC to produce IL-10 in addition to proinflammatory cytokines. IL-12p70 expression in response to CpG was suppressed by endogenous IL-10 in macrophages, in myeloid DC, and to an even greater extent in splenic CD8alpha(-) and CD8alpha(+) DC. Although plasmacytoid DC did not produce IL-10 upon stimulation, addition of this cytokine exogenously suppressed their production of IL-12, TNF, and IFN-alpha, showing trans but not autocrine regulation of these cytokines by IL-10 in plasmacytoid DC. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/67938 |
DOI: | 10.4049/jimmunol.177.11.7551 |
ISSN: | 0022-1767 |
e-ISSN: | 1550-6606 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Boonstra-2006-Macrophages-and-myeloid-dendritic-c.pdf | 951,58 kB | Adobe PDF | Ver/Abrir |