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https://hdl.handle.net/1822/67932
Título: | Myocardium expression of connexin 43, SERCA2a, and myosin heavy chain isoforms are preserved in nitrofen-induced congenital diaphragmatic hernia rat model |
Autor(es): | Baptista, Maria João Recamán, Mónica Rocha, Gustavo Filipe Melo Alves Silva, Cristina Isabel Nogueira Roriz, José-Mário Soares-Fernandes, João Gonzaga, Silvia Santos, Marta Leite-Moreira, Adelino Areias, José Carlos Correia-Pinto, Jorge |
Palavras-chave: | Animals Calcium-Transporting ATPases Connexin 43 Disease Models, Animal Female Fetal Organ Maturity Hernia, Diaphragmatic Hernias, Diaphragmatic, Congenital Myocardium Myosin Heavy Chains Pesticides Phenyl Ethers Pregnancy Protein Isoforms Rats Sarcoplasmic Reticulum Calcium-Transporting ATPases Congenital diaphragmatic hernia Heart SERCA2a Myosin heavy chain isoforms |
Data: | Set-2006 |
Editora: | Elsevier 1 |
Revista: | Journal of Pediatric Surgery |
Citação: | Baptista, M. J., Recamán, M., Melo-Rocha, G., Nogueira-Silva, C., et. al. (2006). Myocardium expression of connexin 43, SERCA2a, and myosin heavy chain isoforms are preserved in nitrofen-induced congenital diaphragmatic hernia rat model. Journal of pediatric surgery, 41(9), 1532-1538 |
Resumo(s): | Background: Previous morphological studies had produced controversial results with regard to heart development in congenital diaphragmatic hernia (CDH), whereas a few publications investigated cardiac function and myocardial maturation. Myocardium maturation is associated with age-dependent increasing of gene expression of gap junction protein connexin 43 (Cx43), adenosine triphosphatase of the sarcoplasmic reticulum (SERCA2a), as well as switching of myosin heavy chains (MHCs) from beta to alpha isoforms. Our aim was to evaluate myocardium maturity in nitrofen-induced CDH rat model.Methods: Fetuses from dated pregnant Sprague-Dawley rats were assigned to 3 experimental groups: control, nitrofen (exposed to nitrofen, without CDH), and CDH (exposed to nitrofen, with CDH). Myocardial samples collected from left ventricle free wall were processed to (i) quantification of messenger RNA (mRNA) of Cx43, SERCA2a, alpha and beta MHC isoforms, as well as beta-actin (housekeeping gene); and (ii) separation of MHC isoforms (a and beta isoforms) by sodium dodecyl sulfate polyacrylamide gel electrophoresis silver stained.Results: We demonstrated that there is no difference in myocardial gene expression of Cx43 (control, 1.0 +/- 0.1; nitrofen, 1.1 +/- 0.2; CDH, 1.3 +/- 0.2) and SERCA2a (control, 1.0 +/- 0.1; nitrofen, 0.9 +/- 0.1; CDH, 1.0 +/- 0.2). Myocardial gene expressions of alpha and beta mRNA of MHC isoforms were slightly decreased both in nitrofen and CDH fetuses when compared with control fetuses, but evaluation of the alpha-to-beta ratios of MHC isoforms at protein level revealed no significant differences between CDH and control (control, 16.9 +/- 2.5; CDH, 17.0 +/- 2.0).Conclusions: Myocardial quantification of Cx43 and ERCA2a mRNA, as well as the expression pattern of MHC isoforms at protein levels, was similar in all studied groups. We predict, therefore, that acute heart failure commonly observed in CDH infants might be attributed predominantly to cardiac overload secondary to severe pulmonary hypertension rathe |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/67932 |
DOI: | 10.1016/j.jpedsurg.2006.05.016 |
ISSN: | 0022-3468 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0022346806003757 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Baptista-2006-Myocardium-expression-of-connexin--.pdf Acesso restrito! | 279,01 kB | Adobe PDF | Ver/Abrir |