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https://hdl.handle.net/1822/6492
Título: | Anatypical active cell death process underlies the fungicidal activity of ciclopiroxolamine against the yeast Saccharomyces cerevisiae |
Autor(es): | Almeida, Bruno Sampaio-Marques, Belém Carvalho, Joana Silva, Manuel T. Leão, Cecília Rodrigues, Fernando José dos Santos Ludovico, Paula |
Palavras-chave: | Ciclopirox olamine Proteases Active cell death Reactive oxygen species Caspase independent caspase-independent cell death |
Data: | 2007 |
Editora: | Oxford University Press |
Revista: | FEMS Yeast Research |
Citação: | Prova tipográfica (In Press) |
Resumo(s): | Ciclopirox olamine (CPO), a fungicidal agent widely used in clinical practice, induced in Saccharomyces cerevisiae an active cell death (ACD) process characterized by changes in nuclear morphology and chromatin condensation associated with the appearance of a population in the sub-G0/G1 cell cycle phase and an arrest in the G2/M phases. This ACD was associated neither with intracellular reactive oxygen species (ROS) signaling, as revealed by the use of different classes of ROS scavengers, nor with a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive phenotype. Furthermore, CPO-induced cell death seems to be dependent on unknown protease activity but independent of the apoptotic regulators Aif1p and Yca1p and of autophagic pathways involving Apg5p, Apg8p and Uth1p. Our results show that CPO triggers in S. cerevisiae an atypical nonapoptotic, nonautophagic ACD with as yet unknown regulators. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/6492 |
DOI: | 10.1111/j.1567-1364.2006.00188.x |
ISSN: | 1567-1356 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
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Anatypical active cell death process underlies the fungicidal activity.pdf | 517,38 kB | Adobe PDF | Ver/Abrir |