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https://hdl.handle.net/1822/64296
Título: | Combination of a peptide-modified gellan gum hydrogel with cell therapy in a lumbar spinal cord injury animal model |
Autor(es): | Gomes, Eduardo D. Mendes, Sofia Silva Leite-Almeida, Hugo Gimble, Jeffrey M. Tam, Roger Y. Shoichet, Molly S. Sousa, Nuno Silva, Nuno André Martins Salgado, A. J. |
Palavras-chave: | Animals Combined Modality Therapy Hydrogels Lumbar Vertebrae Nerve Regeneration Oligopeptides Polysaccharides, Bacterial Rats Rats, Wistar Spinal Cord Injuries Stem Cell Transplantation Treatment Outcome Regenerative medicine Adipose tissue-derived Stromal/Stem Cells Olfactory Ensheathing Cells Extracellular matrix like hydrogels Spinal cord injury Adipose tissue-derived Stromal/Stem Cells Olfactory Ensheathing Cells |
Data: | 2016 |
Editora: | Elsevier 1 |
Revista: | Biomaterials |
Citação: | Gomes, E. D., Mendes, S. S., Leite-Almeida, H., Gimble, J. M., et. al. (2016). Combination of a peptide-modified gellan gum hydrogel with cell therapy in a lumbar spinal cord injury animal model. Biomaterials, 105, 38-51. |
Resumo(s): | Spinal Cord Injury (SCI) is a highly incapacitating condition for which there is still no cure. Current clinical approaches are mainly based on palliative care, so there is a need to find possible treatments to SCI. Cellular transplantation is regarded with great expectation due to the therapeutic potential of cells such as Adipose tissue-derived Stromal/Stem Cells (ASCs) or Olfactory Ensheathing Cells (OECs). Both are accessible sources and present positive paracrine and cell-to-cell interactions, previously reported by our group. Additionally, biomaterials such as hydrogels have been applied in SCI repair with promising results. We propose to combine a GRGDS-modified gellan gum hydrogel with ASCs and OECs in order to promote SCI regeneration. In vitro, ASCs and OECs could be co-cultured within GG-GRGDS hydrogels inducing a more robust neurite outgrowth when compared to controls. In vivo experiments in a hemisection SCI rat model revealed that the administration of ASCs and OECs encapsulated in a GG-GRGDS hydrogel led to significant motor improvements when compared to both control (SCI) and hydrogel alone (GG-GRGDS) groups. This was accompanied by a decreased infiltration of inflammatory cells and astrocytes, and by an increased intensity of neurofilament. These results suggest evident gains induced by the encapsulation of ASCs and OECs in GG-GRGDS based hydrogels. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/64296 |
DOI: | 10.1016/j.biomaterials.2016.07.019 |
ISSN: | 0142-9612 |
e-ISSN: | 1878-5905 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0142961216303507 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Gomes-2016-Combination-of-a-peptide-modified-g.pdf Acesso restrito! | 6,59 MB | Adobe PDF | Ver/Abrir |