Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/62073
Título: | A network approach to discerning the identities of C. elegans in a free moving population |
Autor(es): | Winter, Peter B. Brielmann, Renee M. Timkovich, Nicholas P. Navarro, Helio T. Castro, Andreia Cristiana Teixeira Morimoto, Richard I. Amaral, Luis A. N. |
Palavras-chave: | Animals Caenorhabditis elegans High-Throughput Screening Assays Image Processing, Computer-Assisted Neurons Phenotype Social Behavior Software Behavior, Animal Locomotion |
Data: | 2016 |
Editora: | Nature Research |
Revista: | Scientific Reports |
Citação: | Winter, P. B., Brielmann, R. M., Timkovich, N. P., Navarro, H. T., Teixeira-Castro, A., Morimoto, R. I., & Amaral, L. A. (2016). A network approach to discerning the identities of C. elegans in a free moving population. Scientific reports, 6, 34859. |
Resumo(s): | The study of C. elegans has led to ground-breaking discoveries in gene-function, neuronal circuits, and physiological responses. Subtle behavioral phenotypes, however, are often difficult to measure reproducibly. We have developed an experimental and computational infrastructure to simultaneously record and analyze the physical characteristics, movement, and social behaviors of dozens of interacting free-moving nematodes. Our algorithm implements a directed acyclic network that reconstructs the complex behavioral trajectories generated by individual C. elegans in a free moving population by chaining hundreds to thousands of short tracks into long contiguous trails. This technique allows for the high-throughput quantification of behavioral characteristics that require long-term observation of individual animals. The graphical interface we developed will enable researchers to uncover, in a reproducible manner, subtle time-dependent behavioral phenotypes that will allow dissection of the molecular mechanisms that give rise to organism-level behavior. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/62073 |
DOI: | 10.1038/srep34859 |
ISSN: | 2045-2322 |
e-ISSN: | 2045-2322 |
Versão da editora: | https://www.nature.com/articles/srep34859 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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srep34859.pdf | 1,23 MB | Adobe PDF | Ver/Abrir |