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https://hdl.handle.net/1822/61604
Título: | Cyclooxygenase-2 expression on ifosfamide-induced hemorrhagic cystitis in rats |
Autor(es): | Macedo, Francisco Yuri Bulcao Baltazar, Fátima Almeida, Paulo Roberto Carvalho Távora, Fábio Ferreira, Francisco Valdeci Schmitt, Fernando C. Brito, Gerly Anne Castro Ribeiro, Ronaldo Albuquerque |
Palavras-chave: | Animals Cyclooxygenase 2 Cyclooxygenase 2 Inhibitors Cystitis Drug Therapy, Combination Etoricoxib Hemorrhage Ifosfamide Immunoenzyme Techniques Indomethacin Male Mesna Pentoxifylline Protective Agents Pyridines Rats Rats, Wistar Sulfones Thalidomide Hemorrhagic cystitis TNF-alfa Myofibroblasts TNF-alpha |
Data: | Jan-2008 |
Editora: | Springer |
Revista: | Journal of Cancer Research and Clinical Oncology |
Resumo(s): | Purpose Hemorrhagic cystitis (HC) is a limiting side effect of chemotherapy with ifosfamide (IFS). In this study, we investigated the participation of cyclooxygenase-2 (COX-2) upon ifosfamide-induced HC. Methods Male Wistar rats (150-200 g; six rats per group) were treated with saline, IFS (400 mg/kg, i.p.) and analyzed by changes in bladder wet weight, macroscopic and microscopic parameters, and COX-2 expression. In other groups etoricoxib (selective COX-2 inhibitor), indomethacin (non-selective COX inhibitor), thalidomide (selective TNF-alpha inhibitor), pentoxifyllin (non-selective TNF-alpha inhibitor) were added 1 h before IFS administration. The classical protocol using three doses of Mesna was also evaluated and compared with two extra doses of etoricoxib or indomethacin. Results COX-2 was expressed significantly 24 h after IFS administration mainly in myofibroblasts and mast cells evaluated by immunohistochemistry. Treatment 1 h before IFS injection with etoricoxib, indomethacin, thalidomide, and pentoxifylline reduced COX-2 expression and some macroscopic and microscopic parameters in IFS-induced HC. Moreover, addition of etoricoxib or indomethacin with the last two doses of Mesna was more efficient than three doses of Mesna alone when evaluated microscopically. Conclusions COX-2 participates in the pathogenesis of IFS-induced HC and the treatment with COX and TNF-alpha inhibitors reduced COX-2 expression. The addition of COX-inhibitors to the last two doses of Mesna represents a new therapeutic strategy of preventing HC. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/61604 |
DOI: | 10.1007/s00432-007-0237-6 |
ISSN: | 0171-5216 |
e-ISSN: | 1432-1335 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Macedo-2007-Cyclooxygenase--expression-on-ifosf.pdf Acesso restrito! | 699,3 kB | Adobe PDF | Ver/Abrir |