Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/60971
Título: | BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation |
Autor(es): | Leal, Tânia Abrunhosa, Luís Domingues, Lucília Venâncio, Armando Oliveira, Carla |
Palavras-chave: | Ochratoxin A bovine serum albumin solid-phase extraction wine analysis method validation |
Data: | Dez-2019 |
Editora: | Elsevier 1 |
Revista: | Food Chemistry |
Citação: | Leal, Tânia; Abrunhosa, Luís; Domingues, Lucília; Venâncio, Armando; Oliveira, Carla, BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation. Food Chemistry, 300(125204), 2019 |
Resumo(s): | Analytical chromatographic techniques for mycotoxins control are well established, but they often depend on costly immunoaffinity sample clean-up. Serum albumins, particularly that from bovine origin (BSA), have stable binding affinity towards some mycotoxins, and can be cheaper alternative receptors for sample clean-up due to their wide availability. Thus, this work used BSA immobilized in agarose beads as a novel solid-phase extraction method for quantification of ochratoxin A (OTA) in wine. Constructed BSA-agarose columns could extract OTA efficiently from red wine after its dilution (4-fold) in 0.1 M Tris pH 8.0. The method was linear (R2=0.9999) in the OTA concentration range studied (0.05 to 3.0 g L-1), with recovery rates above 98%. It also showed low detection (0.017 g L-1) and quantification (0.051 g L-1) limits. The efficacy of the BSA-based method was further validated by direct comparison with commercial immunoaffinity columns. Portuguese wines analyzed by both methods had agreeing results. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/60971 |
DOI: | 10.1016/j.foodchem.2019.125204 |
ISSN: | 0308-8146 |
Versão da editora: | http://www.elsevier.com/locate/issn/03088146 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_51852_1.pdf | 440,98 kB | Adobe PDF | Ver/Abrir |