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https://hdl.handle.net/1822/59092
Título: | Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor |
Autor(es): | Pino, Marcela Sofía Rodríguez-Jasso, Rosa M. Michelin, Michele Ruiz, Héctor A. |
Palavras-chave: | Glucan Glucose Biofuels Autohydrolysis Severity Factor Biorefinery |
Data: | 2019 |
Editora: | Elsevier 1 |
Revista: | Carbohydrate Polymers |
Citação: | Pino, Marcela; Rodríguez-Jasso, Rosa M.; Michelin, Michele; Ruiz, Héctor A., Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor. Carbohydrate Polymers, 211, 349-359, 2019 |
Resumo(s): | One of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194°C/30min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6g/L at 72h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/59092 |
DOI: | 10.1016/j.carbpol.2019.01.111 |
ISSN: | 0144-8617 |
e-ISSN: | 0144-8617 |
Versão da editora: | http://www.journals.elsevier.com/carbohydrate-polymers/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_50531_1.pdf | 3,53 MB | Adobe PDF | Ver/Abrir |