Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/57198
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Campo DC | Valor | Idioma |
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dc.contributor.author | Silva, Paulo César | por |
dc.contributor.author | Domingues, Lucília | por |
dc.contributor.author | Collins, Tony | por |
dc.contributor.author | Oliveira, Rui Pedro Soares de | por |
dc.contributor.author | Johansson, Björn | por |
dc.date.accessioned | 2018-12-10T09:57:38Z | - |
dc.date.issued | 2018-12 | - |
dc.identifier.citation | Silva, Paulo César; Domingues, Lucília; Collins, Tony; Oliveira, Rui; Johansson, Björn, Quantitative assessment of DNA damage in the industrial ethanol production strain Saccharomyces cerevisiae PE-2. FEMS Yeast Research, 18(8), foy101, 2018 | por |
dc.identifier.isbn | 1567-1364 | - |
dc.identifier.issn | 1567-1356 | por |
dc.identifier.uri | https://hdl.handle.net/1822/57198 | - |
dc.description.abstract | Lignocellulosic hydrolysates remain one of the most abundantly used substrates for the sustainable production of second generation fuels and chemicals with Saccharomyces cerevisiae. Nevertheless, fermentation inhibitors such as acetic acid, furfural and hydroxymethylfurfural are formed during the process and can lead to slow or stuck fermentations and/or act as genotoxic agents leading to production strain genetic instability. We have developed a novel dominant deletion (DEL) cassette assay for quantification of DNA damage in both wild-type and industrial yeast strains. Using this assay, the ethanol production strain S. cerevisiae PE-2 was shown to be more resistant to hydrogen peroxide and furfural than the laboratory DEL strain RS112. Indeed, the PE-2 strain also showed a lower tendency for recombination, consistent with a more efficient DNA protection. The dominant DEL assay presented herein should prove to be a useful tool in the selection of robust yeast strains and process conditions for second generation feedstock fermentations. | por |
dc.description.sponsorship | This work was supported by the Fundação para a Ciência e a Tecnologia Portugal (FCT) through Project MycoFat PTDC/AAC-AMB/120940/2010. CBMA was supported by the strategic programme UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) funded by national funds through the FCT I.P. and by the ERDF through the COMPETE2020—Programa Operacional Competitividade e Internacionalizacao (POCI). This work is supported by: European Investment Funds by FEDER/COMPETE/POCI— Operational Competitiveness and Internationalization Programme, under Project POCI-01-0145-FEDER-006958 and National Funds by FCT—Portuguese Foundation for Science and Technology, under the project UID/AGR/04033/2013. T. Collins thanks the FCT for support through the Investigador FCT Programme (IF/01635/2014). | por |
dc.language.iso | eng | por |
dc.publisher | Oxford University Press | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/120940/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/120940/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147341/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | industrial Saccharomyces cerevisiae strain | por |
dc.subject | genotoxicity | por |
dc.subject | yeast background | por |
dc.subject | fermentation inhibitor | por |
dc.subject | furfural | por |
dc.subject | genetic stability | por |
dc.title | Quantitative assessment of DNA damage in the industrial ethanol production strain Saccharomyces cerevisiae PE-2 | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://femsyr.oxfordjournals.org/ | por |
dc.comments | CEB49086 | por |
oaire.citationStartPage | foy101 | por |
oaire.citationIssue | 8 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationVolume | 18 | por |
dc.date.updated | 2018-12-09T13:15:50Z | - |
dc.identifier.eissn | 1567-1364 | por |
dc.identifier.doi | 10.1093/femsyr/foy101 | por |
dc.identifier.pmid | 30219865 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | FEMS Yeast Research | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DBio - Artigos/Papers |
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document_49086_1.pdf Acesso restrito! | 704,12 kB | Adobe PDF | Ver/Abrir |