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https://hdl.handle.net/1822/57041
Título: | Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization |
Autor(es): | Faria, Cristiana Borges, Nuno Rocha, I. Santos, Helena |
Palavras-chave: | Mannosylglycerate Compatible solute GDP-mannose Yeast cell factory Metabolic engineering Chemostat cultivation |
Data: | 16-Nov-2018 |
Editora: | Springer Nature |
Revista: | Microbial Cell Factories |
Citação: | Faria, Cristiana; Borges, Nuno; Rocha, Isabel; Santos, Helena, Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization. Microbial Cell Factories, 17(1), 178, 2018 |
Resumo(s): | Mannosylglycerate (MG) is one of the most widespread compatible solutes among marine microorganisms adapted to hot environments. This ionic solute holds excellent ability to protect proteins against thermal denaturation, hence a large number of biotechnological and clinical applications have been put forward. However, the current prohibitive production costs impose severe constraints towards large-scale applications. All known microbial producers synthesize MG from GDP-mannose and 3-phosphoglycerate via a two-step pathway in which mannosyl-3-phosphoglycerate is the intermediate metabolite. In an early work, this pathway was expressed in Saccharomyces cerevisiae with the goal to confirm gene function (Empadinhas et al. in J Bacteriol 186:4075--4084, 2004), but the level of MG accumulation was low. Therefore, in view of the potential biotechnological value of this compound, we decided to invest further effort to convert S. cerevisiae into an efficient cell factory for MG production. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/57041 |
DOI: | 10.1186/s12934-018-1023-7 |
ISSN: | 1475-2859 |
e-ISSN: | 1475-2859 |
Versão da editora: | http://www.microbialcellfactories.com/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_49167_1.pdf | 1,1 MB | Adobe PDF | Ver/Abrir |