Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/51751
Título: | Yeast Pathway Kit: A Method for Metabolic Pathway Assembly with Automatically Simulated Executable Documentation |
Autor(es): | Pereira, Filipa Alexandra Barroso Azevedo, Flávio Humberto Torres Dias Feio Parachin, Nadia Skorupa Hahn-Hagerdal, Barbel Gorwa-Grauslund, Marie F. Johansson, Björn |
Palavras-chave: | Metabolic engineering Saccharomyces cerevisiae D-xylose Synthetic biology Bioinformatics |
Data: | 1-Mai-2016 |
Editora: | ACS |
Revista: | ACS SYNTHETIC BIOLOGY |
Citação: | Pereira, F., Azevedo, F., Parachin, N. S., Hahn-Hägerdal, B., Gorwa-Grauslund, M. F., & Johansson, B. (2016). Yeast pathway kit: a method for metabolic pathway assembly with automatically simulated executable documentation. ACS synthetic biology, 5(5), 386-394 |
Resumo(s): | We have developed the Yeast Pathway Kit (YPK) for rational and random metabolic pathway assembly in Saccharomyces cerevisiae using reusable and redistributable genetic elements. Genetic elements are cloned in a suicide vector in a rapid process that omits PCR product purification. Single-gene expression cassettes are assembled in vivo using genetic elements that are both promoters and terminators (TP). Cassettes sharing genetic elements are assembled by recombination into multigene pathways. A wide selection of prefabricated TP elements makes assembly both rapid and inexpensive. An innovative software tool automatically produces detailed self-contained executable documentation in the form of pydna code in the narrative Jupyter notebook format to facilitate planning and sharing YPK projects. A D-xylose catabolic pathway was created using YPK with four or eight genes that resulted in one of the highest growth rates reported on D-xylose (0.18 h(-1)) for recombinant S. cerevisiae without adaptation. The two-step assembly of single-gene expression cassettes into multigene pathways may improve the yield of correct pathways at the cost of adding overall complexity, which is offset by the supplied software tool. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/51751 |
DOI: | 10.1021/acssynbio.5b00250 |
ISSN: | 2161-5063 |
Versão da editora: | https://pubs.acs.org/doi/abs/10.1021/acssynbio.5b00250 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Pereira et al. 2016 b.pdf Acesso restrito! | 1,67 MB | Adobe PDF | Ver/Abrir |