Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/51593
Título: | Colony PCR |
Autor(es): | Azevedo, Flávio Pereira, Humberto Johansson, Björn |
Palavras-chave: | Colony Direct lysis Escherichia coli PCR Saccharomyces cerevisiae Yeast |
Data: | 1-Jan-2017 |
Editora: | Springer |
Revista: | Methods in Molecular Biology |
Citação: | Azevedo F., Pereira H., Johansson B. (2017) Colony PCR. In: Domingues L. (eds) PCR. Methods in Molecular Biology, vol 1620. Springer, New York, NY |
Resumo(s): | Escherichia coli and Saccharomyces cerevisiae are currently the two most important organisms in synthetic biology. E.coli is almost always used for fundamental DNA manipulation while yeast is the simplest host system for studying eukaryotic gene expression and performing large scale DNA assembly. Yeast expression studies may also require altering of the chromosomal DNA by homologous recombination. All these studies require the verification of the expected DNA sequence and the fastest method of screening is colony PCR, which is direct PCR of DNA in cells without prior DNA purification. Colony PCR is hampered by the difficulty of releasing DNA into the PCR mix and the presence of PCR inhibitors. We hereby present one protocol for E. coli and two protocols for S. cerevisiae differing in efficiency and complexity as well as an overview of past and possible future developments of efficient S. cerevisiae colony PCR protocols. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/51593 |
ISBN: | 978-1-4939-7059-9 |
e-ISBN: | 978-1-4939-7060-5 |
DOI: | 10.1007/978-1-4939-7060-5_8 |
ISSN: | 1064-3745 |
Versão da editora: | https://link.springer.com/protocol/10.1007/978-1-4939-7060-5_8#citeas |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
Aparece nas coleções: | DBio - Livros e Capítulos de Livros/Books and Books Chapters |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Azevedo 2017.pdf Acesso restrito! | 211,31 kB | Adobe PDF | Ver/Abrir |