Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/49479
Título: | New ß-galactosidase producers with potential for prebiotic synthesis |
Autor(es): | Silvério, Sara Isabel Cruz Macedo, Eugénia A. Teixeira, J. A. Rodrigues, L. R. |
Palavras-chave: | ß-galactosidase fungal strains lactulose galacto-oligosaccharides fructo-oligosaccharides β-Galactosidase |
Data: | Fev-2018 |
Editora: | Elsevier 1 |
Revista: | Bioresource Technology |
Citação: | Silvério, Sara C.; Macedo, Eugénia A.; Teixeira, José A.; Rodrigues, Lígia R., New ß-galactosidase producers with potential for prebiotic synthesis. Bioresource Technology, 250, 131-139, 2018 |
Resumo(s): | ß-galactosidases (EC 3.2.1.23) are interesting enzymes with potential application in the pharmaceutical and food industry. In this work, a screening study was carried out to identify new fungal sources of -galactosidase. A total of 50 fungi were evaluated using a chromogenic test performed in agar plates. The most promising microorganisms were validated as effective -galactosidase producers under submerged fermentation conditions. The crude -galactosidases were characterized regarding their optimal pH (3.0-5.5) and temperature (45-65 °C). All enzymes showed ability to synthesize lactose-based prebiotics, namely lactulose (maximal yield 3.3%) and a galacto-oligosaccharide (GOS) (maximal yield 20%). Additionally, some enzymatic extracts with fructosyltransferase activity allowed to produce other type of prebiotics, namely fructo-oligosaccharides (FOS). This work, reports for the first time the simultaneous synthesis of different mixtures of GOS (2-15% yield and 0.07-0.5 g/L·h-1 productivity) and FOS (4-30% yield and 0.1-1 g/L·h-1 productivity) by crude extracts exhibiting dual enzymatic activity. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/49479 |
DOI: | 10.1016/j.biortech.2017.11.045 |
ISSN: | 0960-8524 |
e-ISSN: | 0960-8524 |
Versão da editora: | http://www.journals.elsevier.com/bioresource-technology/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_47252_1.pdf | 358,46 kB | Adobe PDF | Ver/Abrir |