Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/45062
Título: | A low-cost HPV immunochromatographic assay to detect high-grade cervical intraepithelial neoplasia |
Autor(es): | Mariano, Vânia Sammartino Lorenzi, Adriana Tarlá Scapulatempo-Neto, Cristovam Stein, Maíra Degiovani Resende, Julio Cesar Possati Antoniazzi, Márcio Villa, Luisa Lina Levi, José Eduardo Longatto Filho, Adhemar Fregnani, José Humberto Tavares Guerreiro |
Data: | Out-2016 |
Editora: | Public Library of Science |
Revista: | PLoS ONE |
Citação: | Mariano, V. S., Lorenzi, A. T., Scapulatempo-Neto, C., Stein, M. D., Resende, J. C. P., Antoniazzi, M., et. al.(2016). A Low-Cost HPV Immunochromatographic Assay to Detect High-Grade Cervical Intraepithelial Neoplasia. PloS one, 11(10), e0164892 |
Resumo(s): | Objective To evaluate the reproducibility and accuracy of the HPV16/18-E6 test. Methods The study population was comprised of 448 women with a previously abnormal Pap who were referred to the Barretos Cancer Hospital (Brazil) for diagnosis and treatment. Two cervical samples were collected immediately before colposcopy, one for the hr-HPV-DNA test and cytology and the other for the HPV16/18-E6 test using high-affinity monoclonal antibodies (mAb). Women with a histologic diagnosis of cervical intraepithelial neoplasia grade 2 or 3 were considered to be positive cases. Different strategies using a combination of screening methods (HPV-DNA) and triage tests (cytology and HPV16/18-E6) were also examined and compared. Results The HPV16/18-E6 test exhibited a lower positivity rate compared with the HPV-DNA test (19.0% vs. 29.3%, p<0.001) and a moderate/high agreement (kappa = 0.68, 95% CI: 0.60-0.75). It also exhibited a significantly lower sensitivity for CIN2+ and CIN3+ detection compared to the HPV-DNA test and a significantly higher specificity. The HPV16/18-E6 test was no different from cytology in terms of sensitivity, but it exhibited a significantly higher specificity in comparison to ASCH+. A triage test after HPV-DNA detection using the HPV16/18-E6 test exhibited a significantly higher specificity compared with a triage test of ASCH+ to CIN2+ (91.8% vs. 87.4%, p = 0.04) and CIN3+ (88.6% vs. 84.0%, p = 0.05). Conclusion The HPV16/18-E6 test exhibited moderate/high agreement with the HPV-DNA test but lower sensitivity and higher specificity for the detection of CIN2+ and CIN3+. In addition, its performance was quite similar to cytology, but because of the structural design addressed for the detection of HPV16/18-E6 protein, the test can miss some CIN2/3+ lesions caused by other high-risk HPV types. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/45062 |
DOI: | 10.1371/journal.pone.0164892 |
ISSN: | 1932-6203 |
Versão da editora: | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0164892 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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journal.pone.0164892.pdf | 1,32 MB | Adobe PDF | Ver/Abrir |