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https://hdl.handle.net/1822/43915
Título: | Protective effect of antigen delivery using monoolein-based liposomes in experimental hematogenously disseminated candidiasis |
Autor(es): | Carneiro, Catarina Correia, Alexandra Lima, Tanea Vilanova, Manuel Pais, Célia Gomes, Andreia C Real Oliveira, M. Elisabete C.D. Sampaio, Paula |
Palavras-chave: | DODAB:MO liposomes Antigen delivery systems Candida albicans proteins Dendritic cells Nanoparticles Humoral and cellular immune responses |
Data: | 1-Mai-2016 |
Editora: | Elsevier 1 |
Revista: | Acta Biomaterialia |
Citação: | Acta Biomaterialia 39 (2016) 133–145 |
Resumo(s): | We evaluated the potential of a liposomal antigen delivery system (ADS) containing Candida albicans cell wall surface proteins (CWSP) in mediating protection against systemic candidiasis. Treatment of bonemarrow- derived dendritic cells with CWSP-loaded dioctadecyldimethylammonium bromide:monoolein (DODAB:MO) liposomes enhanced and prolonged their activation comparatively to free antigen, indicating that liposome-entrapped CWSP were released more sustainable. Therefore, we immunized mice with CWSP either in a free form or loaded into two different DODAB:MO liposome formulations, respectively designated as ADS1 and ADS2, prior to intravenous C. albicans infection. Immunization with ADS1, but not with ADS2, conferred significant protection to infected mice, comparatively to immunization with CWSP or empty liposomes as control. ADS1-immunized mice presented significantly higher serum levels of C. albicans-specific antibodies that enhanced phagocytosis of this fungus. In these mice, a mixed cytokine production profile was observed encompassing IFN-c, IL-4, IL-17A and IL-10. Nevertheless, only production of IL-4, IL-17 and IL-10 was higher than in controls. In this study we demonstrated that DODAB:MO liposomes enhance the immunogenicity of C. albicans antigens and host protection in a murine model of systemic candidiasis. Therefore, this liposomal adjuvant could be a promising candidate to assess in vaccination against this pathogenic fungus. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/43915 |
DOI: | 10.1016/j.actbio.2016.05.001 |
ISSN: | 1742-7061 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CBMA - Artigos/Papers CDF - FAMO - Artigos/Papers (with refereeing) DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Carneiro C et al. abiomat 2016.pdf | 2,06 MB | Adobe PDF | Ver/Abrir |