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https://hdl.handle.net/1822/42900
Título: | Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation |
Autor(es): | Ramos, Philippe Emmanuel Cruz Abrunhosa, Luís Pinheiro, Ana Cristina Cerqueira, Miguel A. Motta, Carla Castanheira, Isabel Chandra-Hioe, Maria V. Arcot, Jayashree Teixeira, J. A. Vicente, A. A. |
Palavras-chave: | Probiotic Immobilization 5-Methyltetrahydrofolate Mucoadhesive Folic acid |
Data: | Dez-2016 |
Editora: | Elsevier 1 |
Revista: | Food Research International |
Citação: | Ramos, Philippe E.; Abrunhosa, Luís; Pinheiro, Ana Cristina; Cerqueira, Miguel A.; Motta, Carla; Castanheira, Isabel; Chandra-Hioe, Maria V.; Arcot, Jayashree; Teixeira, J. A.; Vicente, António A., Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation. Food Research International, 90, 25-32, 2016 |
Resumo(s): | This study focused on the use of a new system, an alginate | -poly-l-lysine | alginate | chitosan microcapsule (APACM), able to immobilize a folate-producing probiotic, Lactococcus lactis ssp. cremoris (LLC), which provides a new approach to the utilization of capsules and probiotics for in situ production of vitamins. LLC is able to produce 95.25 ± 26 g·L 1 of folate, during 10 h, and was encapsulated in the APACM. APACM proved its capacity to protect LLC against the harsh conditions of a simulated digestion maintaining a viable concentration of 6 log CFU·mL 1of LLC. A nutrients exchange capacity test, was performed using Lactobacillus plantarum UM7, a high lactic acid producer was used here to avoid false negative results. The production and release of 2 g·L 1 of lactic acid was achieved through encapsulation of L. plantarum, after 20 h. The adhesion of APACM to epithelial cells was also quantified, yielding 38% and 33% of capsules adhered to HT-29 cells and Caco-2 cells, respectively. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/42900 |
DOI: | 10.1016/j.foodres.2016.10.036 |
ISSN: | 0963-9969 |
Versão da editora: | http://www.sciencedirect.com/science/article/pii/S0963996916304781 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_46223_1.pdf | 869,36 kB | Adobe PDF | Ver/Abrir |