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https://hdl.handle.net/1822/42721
Título: | MERVL/Zscan4 network activation results in transient genome-wide DNA demethylation of mESCs |
Autor(es): | Eckersley-Maslin, Mélanie A. Svensson, Valentine Krueger, Christel Stubbs, Thomas M. Giehr, Pascal Krueger, Felix Miragaia, Ricardo Júdice Kyriakopoulos, Charalampos Berrens, Rebecca V. Milagre, Inês Walter, Jörn Teichmann, Sarah A. Reik, Wolf |
Palavras-chave: | Zscan4 MERVL Endogenous retrovirus Embryonic stem cell DNA methylation Imprint Preimplantation Reprogramming Chromatin |
Data: | 2016 |
Editora: | Cell Press |
Revista: | Cell Reports |
Citação: | Eckersley-Maslin, Mélanie A.; Svensson, Valentine; Krueger, Christel; Stubbs, Thomas M.; Giehr, Pascal; Krueger, Felix; Miragaia, R.; Kyriakopoulos, Charalampos; Berrens, Rebecca V.; Milagre, Inês; Walter, Jörn; Teichmann, Sarah A.; Reik, Wolf, MERVL/Zscan4 network activation results in transient genome-wide DNA demethylation of mESCs. Cell Reports, 17(1), 179-192, 2016 |
Resumo(s): | Mouse embryonic stem cells are dynamic and heterogeneous. For example, rare cells cycle through a state characterized by decondensed chromatin and expression of transcripts, including the Zscan4 cluster and MERVL endogenous retrovirus, which are usually restricted to preimplantation embryos. Here, we further characterize the dynamics and consequences of this transient cell state. Single-cell transcriptomics identified the earliest upregulated transcripts as cells enter the MERVL/Zscan4 state. The MERVL/Zscan4 transcriptional network was also upregulated during induced pluripotent stem cell reprogramming. Genome-wide DNA methylation and chromatin analyses revealed global DNA hypomethylation accompanying increased chromatin accessibility. This transient DNA demethylation was driven by a loss of DNA methyltransferase proteins in the cells and occurred genome-wide. While methylation levels were restored once cells exit this state, genomic imprints remained hypomethylated, demonstrating a potential global and enduring influence of endogenous retroviral activation on the epigenome. |
Tipo: | Artigo |
Descrição: | Supplemental Information includes Supplemental Experimental Procedures, six figures, and four tables and can be found with this article online at http:// dx.doi.org/10.1016/j.celrep.2016.08.087. |
URI: | https://hdl.handle.net/1822/42721 |
DOI: | 10.1016/j.celrep.2016.08.087 |
ISSN: | 2211-1247 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_45182_1.pdf | 4,48 MB | Adobe PDF | Ver/Abrir |