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https://hdl.handle.net/1822/42546
Título: | Current state and challenges for dynamic metabolic modeling |
Autor(es): | Vasilakou, Eleni Machado, Daniel Theorell, Axel Rocha, Isabel Nöh, Katharina Oldiges, Marco Wahl, S. Aljoscha |
Data: | Out-2016 |
Editora: | Elsevier B.V. |
Revista: | Current Opinion in Microbiology |
Citação: | Vasilakou, Eleni; Machado, Daniel; Theorell, Axel; Rocha, Isabel; Nöh, Katharina; Oldiges, Marco; Wahl, S. Aljoscha, Current state and challenges for dynamic metabolic modeling. Current Opinion in Microbiology, 33, 97-104, 2016 |
Resumo(s): | While the stoichiometry of metabolism is probably the best studied cellular level, the dynamics in metabolism can still not be well described, predicted and, thus, engineered. Unknowns in the metabolic flux behavior arise from kinetic interactions, especially allosteric control mechanisms. While the stoichiometry of enzymes is preserved in vitro, their activity and kinetic behavior differs from the in vivo situation. Next to this challenge, it is infeasible to test the interaction of each enzyme with each intracellular metabolite in vitro exhaustively. As a consequence, the whole interacting metabolome has to be studied in vivo to identify the relevant enzymes properties. In this review we discuss current approaches for in vivo perturbation experiments, that is, stimulus response experiments using different setups and quantitative analytical approaches, including dynamic carbon tracing. Next to reliable and informative data, advanced modeling approaches and computational tools are required to identify kinetic mechanisms and their parameters. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/42546 |
DOI: | 10.1016/j.mib.2016.07.008 |
ISSN: | 1369-5274 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_40109_1.pdf | 521,61 kB | Adobe PDF | Ver/Abrir |