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https://hdl.handle.net/1822/42183
Título: | A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex |
Autor(es): | Villani, Alessandra Moretti, Antonio De Saeger, Sarah Han, Zheng Di Mavungu, Jose Diana Soares, Célia Maria Gonçalves Proctor, Robert H. Venâncio, Armando Lima, Nelson Stea, Gaetano Paciolla, Costantino Logrieco, Antonio F. Susca, Antonia |
Palavras-chave: | FIESC Secondary metabolites Phylogeny TRI genes |
Data: | 2016 |
Editora: | Elsevier 1 |
Revista: | International Journal of Food Microbiology |
Citação: | Villani, Alessandra; Moretti, Antonio; De Saeger, Sarah; Han, Zheng; Di Mavungu, Jose Diana; Soares, C.; Proctor, Robert H.; Venâncio, Armando; Lima, Nelson; Stea, Gaetano; Paciolla, Costantino; Logrieco, Antonio F.; Susca, Antonia, A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex. International Journal of Food Microbiology, 234, 24-35, 2016 |
Resumo(s): | DNA-based phylogenetic analyses have resolved the fungal genus Fusarium into multiple species complexes. The F. incarnatum-equiseti species complex (FIESC) includes fusaria associated with several diseases of agriculturally important crops, including cereals. Although members of FIESC are considered to be only moderately aggressive, they are able to produce a diversity of mycotoxins, including trichothecenes, which can accumulate to harmful levels in cereals. High levels of cryptic speciation have been detected within the FIESC. As a result, it is often necessary to use approaches other than morphological characterization to distinguish species. In the current study, we used a polyphasic approach to characterize a collection of 69 FIESC isolates recovered from cereals in Europe, Turkey, and North America. In a species phylogeny inferred from nucleotide sequences from four housekeeping genes, 65 of the isolates were resolved within the Equiseti clade of the FIESC, and four isolates were resolved within the Incarnatum clade. Seven isolates were resolved as a genealogically exclusive lineage, designated here as FIESC 31. Phylogenies based on nucleotide sequences of trichothecene biosynthetic genes and MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry) were largely concordant with phylogeny inferred from the housekeeping gene. Finally, Liquid Chromatography (Time-Of-Flight) Mass Spectrometry [LC-(TOF-)MS(/MS)] revealed variability in mycotoxin production profiles among the different phylogenetic species investigated in this study. |
Tipo: | Artigo |
Descrição: | "Available online 22 June 2016" |
URI: | https://hdl.handle.net/1822/42183 |
DOI: | 10.1016/j.ijfoodmicro.2016.06.023 |
ISSN: | 0168-1605 |
e-ISSN: | 0168-1605 |
Versão da editora: | http://www.journals.elsevier.com/international-journal-of-food-microbiology/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_40043_1.pdf | 1,15 MB | Adobe PDF | Ver/Abrir |