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https://hdl.handle.net/1822/42056
Título: | Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium |
Autor(es): | Muñiz-Márquez, Diana B. Contreras, Juan C. Rodríguez, Raúl Mussatto, Solange I. Teixeira, J. A. Aguilar, Cristóbal N. |
Palavras-chave: | Fructosyltransferase Fructooligosaccharides Aguamiel Solid State Fermentation |
Data: | Jun-2016 |
Editora: | Elsevier 1 |
Revista: | Bioresource Technology |
Citação: | Muñiz-Márquez, Diana B.; Contreras, Juan C.; Rodríguez, Raúl; Mussatto, Solange I.; Teixeira, J. A.; Aguilar, Cristóbal N., Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium. Bioresource Technology, 213, 276-282, 2016 |
Resumo(s): | The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347 U/L) was obtained at 32 °C, using packing density of 0.7 g/cm3. Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/42056 |
DOI: | 10.1016/j.biortech.2016.03.022 |
ISSN: | 0960-8524 |
e-ISSN: | 0960-8524 |
Versão da editora: | http://www.journals.elsevier.com/bioresource-technology/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_37923_1.pdf | 790,92 kB | Adobe PDF | Ver/Abrir |