Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/37008

TitleDiels–Alder functionalized carbon nanotubes for bone tissue engineering : in vitro/in vivo biocompatibility and biodegradability
Author(s)Mata, Diogo
Amaral, M.
Fernandes, A. J. S.
Colaço, B.
Gama, A.
Paiva, M. C.
Gomes, P. S.
Silva, R. F.
Fernandes, M. H.
Issue date2015
PublisherRoyal Society of Chemistry
JournalNanoscale
Citation7, 20 (2015) 9238-9251
Abstract(s)The risk-benefit balance for carbon nanotubes (CNTs) dictates their clinical fate. To take a step forward at this crossroad it is compulsory to modulate the CNT in vivo biocompatibility and biodegradability via e.g. chemical functionalization. CNT membranes were functionalised combining a Diels–Alder cycloaddition reaction to generate cyclohexene (–C6H10) followed by a mild oxidisation to yield carboxylic acid groups (–COOH). In vitro proliferation and osteogenic differentiation of human osteoblastic cells were maximized on functionalized CNT membranes (p,f-CNTs). The in vivo subcutaneously implanted materials showed a higher biological reactivity, thus inducing a slighter intense inflammatory response compared to nonfunctionalized CNT membranes (p-CNTs), but still showing a reduced cytotoxicity profile. Moreover, the in vivo biodegradation of CNTs was superior for p,f-CNT membranes, likely mediated by the oxidationinduced myeloperoxidase (MPO) in neutrophil and macrophage inflammatory milieus. This proves the biodegradability faculty of functionalized CNTs, which potentially avoids long-term tissue accumulation and triggering of acute toxicity. On the whole, the proposed Diels–Alder functionalization accounts for the improved CNT biological response in terms of the biocompatibility and biodegradability profiles. Therefore, CNTs can be considered for use in bone tissue engineering without notable toxicological threats.
TypeArticle
URIhttp://hdl.handle.net/1822/37008
DOI10.1039/c5nr01829c
ISSN2040-3364
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:IPC - Artigos em revistas científicas internacionais com arbitragem

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