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https://hdl.handle.net/1822/34586
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Carvalhais, Virgínia Maria Dinis | por |
dc.contributor.author | Cerveira, Frederico | por |
dc.contributor.author | Vilanova, Manuel | por |
dc.contributor.author | Cerca, Nuno | por |
dc.contributor.author | Vitorino, Rui | por |
dc.date.accessioned | 2015-03-27T14:03:44Z | - |
dc.date.available | 2015-03-27T14:03:44Z | - |
dc.date.issued | 2015-06 | - |
dc.identifier.citation | Carvalhais, V.; Cerveira, F.; Vilanova, M.; Cerca, Nuno; Vitorino, R., An immunoproteomic approach for characterization of dormancy within Staphylococcus epidermidis biofilms. Molecular Immunology, 65(2), 429-435, 2015 | por |
dc.identifier.issn | 0161-5890 | por |
dc.identifier.uri | https://hdl.handle.net/1822/34586 | - |
dc.description.abstract | Virulence of Staphylococcus epidermidis is mainly attributed to surface colonization and biofilm formation in indwelling medical devices. Physiological heterogeneity of biofilms may influence host immune response and sensitivity to antibiotics. Dormant cells, among others, contribute to biofilm heterogeneity. The aim of this study was to identify immunogenic proteins of S. epidermidis biofilms associated with dormancy mechanism, by using two-dimensional electrophoresis (2-DE) immunoblotting and mass spectrometry (MS). A total of 19 bacterial proteins, recognized by human serum samples, were identified. These proteins were mainly involved in small molecule metabolic biological processes. Catalytic activity and ion binding were the most representative molecular functions. CodY and GpmA proteins were more reactive to sera when biofilm dormancy was induced, while FtnA and ClpP were more reactive when dormancy was prevented. This is the first work that identifies differences in immunoreactive proteins within bacterial biofilms with induced or prevented dormancy. Considering the importance of dormancy within biofilms, further evaluation of these proteins can provide insights into the mechanisms related to dormancy and help to improve current understanding on how dormancy affects the host immune response. | por |
dc.description.sponsorship | VC had an individual FCT fellowship (SFRH/BD/78235/2011). NC is an Investigator FCT. This work was funded by Fundacao para a Ciencia e a Tecnologia (FCT) and COMPETE grants PTDC/BIA-MIC/113450/2009, FCOMP-01-0124-FEDER-014309, QOPNA research unit (project PEst-C/QUI/UI0062/2013), RNEM (National Mass Spetrometry Network) and CENTRO-07-ST24-FEDER-002034. The authors also thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013 and the Project "BioHealth - Biotechnology and Bioengineering approaches to improve health quality", Ref. NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2 - 0 Novo Norte), QREN, FEDER. The authors also acknowledge the project "Consolidating Research Expertise and Resources on Cellular and Molecular Biotechnology at CEB/IBB", Ref. FCOMP-01-0124-FEDER-027462. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/113450/PT | - |
dc.rights | openAccess | por |
dc.subject | S. epidermidis biofilm | por |
dc.subject | Dormancy | por |
dc.subject | Immunoproteomics | por |
dc.title | An immunoproteomic approach for characterization of dormancy within Staphylococcus epidermidis biofilms | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.elsevier.com/locate/molimm | por |
dc.comments | CEB19848 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 429 | por |
oaire.citationEndPage | 435 | por |
oaire.citationIssue | 2 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationTitle | Molecular immunology | por |
oaire.citationVolume | 65 | por |
dc.date.updated | 2015-03-10T22:33:25Z | - |
dc.identifier.eissn | 0161-5890 | - |
dc.identifier.doi | 10.1016/j.molimm.2015.02.024 | por |
dc.identifier.pmid | 25749707 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Molecular immunology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_19848_1.pdf | 1,18 MB | Adobe PDF | Ver/Abrir |