Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/33166
Título: | Evaluation of the best method to assess antibiotic potentiation by phytochemicals against staphylococcus aureus |
Autor(es): | Abreu, Ana Cristina Serra, Sofia Cristina Borges, Anabela Saavedra, Maria José Salgado, A. J. Simões, Manuel |
Palavras-chave: | Alkaloids Co-therapy Efflux pump Potentiation Resistance-modifying agents |
Data: | Jun-2014 |
Editora: | Elsevier 1 |
Revista: | Diagnostic Microbiology and Infectious Disease |
Resumo(s): | The increasing occurrence of bacterial resistance to antibiotics has now reached a critical level. Finding antibiotic coadjuvants capable to inhibit the bacterial resistance mechanisms would be a valuable mid-term solution, until new classes of antibiotics are discovered. Selected plant alkaloids were combined with 5 antibiotics against 10 Staphylococcus aureus strains, including strains expressing distinct efflux pumps and methicillin-resistant S. aureus strains. The efficacy of each combination was assessed using the microdilution checkerboard, time-kill, Etest, and disc diffusion methods. The cytotoxicity of the alkaloids was evaluated in a mouse fibroblast cell line. Potentiation was obtained in 6% of all 190 combinations, especially with the combination of: ciprofloxacin with reserpine (RES), pyrrolidine (PYR), and quinine (QUIN); tetracycline with RES; and erythromycin with PYR. The highest cytotoxicity values were found for QUIN (half maximal inhibitory concentration [IC50] = 25 ± 2.2 mg/L) and theophylline (IC50 = 100 ± 4.7 mg/L). |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/33166 |
DOI: | 10.1016/j.diagmicrobio.2014.03.002 |
ISSN: | 0732-8893 |
Versão da editora: | http://www.springer.com |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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abreu ac_diagn micr infec dis 2014 posp.pdf | 584,88 kB | Adobe PDF | Ver/Abrir |