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TitleMetabolic engineering of Saccharomyces cerevisiae ethanol strains PE-2 and CAT-1 for efficient lignocellulosic fermentation
Author(s)Romaní, Aloia
Pereira, Filipa
Johansson, Björn
Domingues, Lucília
KeywordsSecond generation bio-ethanol
Xylose fermentation
GRE3 aldose reductase
Industrial Saccharomyces cerevisiae
Simultaneous saccharification and co-fermentation (SSCF)
Issue date2015
PublisherElsevier B.V.
JournalBioresource technology
CitationRomaní, Aloia; Pereira, Filipa; Johansson, Björn; Domingues, Lucília, Metabolic engineering of Saccharomyces cerevisiae ethanol strains PE-2 and CAT-1 for efficient lignocellulosic fermentation. Bioresource Technology, 179, 150-158, 2015.
Abstract(s)In this work, Saccharomyces cerevisiae strains PE-2 and CAT-1, commonly used in the Brazilian fuel ethanol industry, were engineered for xylose fermentation, where the first fermented xylose faster than the latter, but also produced considerable amounts of xylitol. An engineered PE-2 strain (MEC1121) efficiently consumed xylose in presence of inhibitors both in synthetic and corn-cob hydrolysates. Interestingly, the S. cerevisiae MEC1121 consumed xylose and glucose simultaneously, while a CEN.PK based strain consumed glucose and xylose sequentially. Deletion of the aldose reductase GRE3 lowered xylitol production to undetectable levels and increased xylose consumption rate which led to higher final ethanol concentrations. Fermentation of corn-cob hydrolysate using this strain, MEC1133, resulted in an ethanol yield of 0.47 g/g of total sugars which is 92% of the theoretical yield.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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