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https://hdl.handle.net/1822/31731
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Campo DC | Valor | Idioma |
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dc.contributor.author | Portela, Carla | por |
dc.contributor.author | Smart, Kathleen F. | por |
dc.contributor.author | Tumanov, Sergey | por |
dc.contributor.author | Cook, Gregory M. | por |
dc.contributor.author | Villas-Bôas, S. G. | por |
dc.date.accessioned | 2014-12-09T13:20:30Z | - |
dc.date.available | 2014-12-09T13:20:30Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1098-5530 | por |
dc.identifier.uri | https://hdl.handle.net/1822/31731 | - |
dc.description.abstract | Oxygen and oxidative stress have become relevant components in clarifying the mechanism that weakens bacterial cells in parallel to the mode of action of bactericidal antibiotics. Given the importance of oxidative stress in the overall defense mechanism of bacteria and their apparent role in the antimicrobial mode of action, it is important to understand how bacteria respond to this stress at a metabolic level. The aim of this study was to determine the impact of oxygen on the metabolism of the facultative anaerobe Enterococcus faecalis using continuous culture, metabolomics and 13C-enrichment of metabolic intermediates. When E. faecalis was rapidly transitioned from anaerobic to aerobic growth, cellular metabolism was directed towards intracellular glutathione production and glycolysis was upregulated two-fold, which increased the supply of critical metabolite precursors (e.g. glycine and glutamate) for sulfur metabolism and glutathione biosynthesis as well as reducing power for cellular respiration in the presence of haemin. The ultimate metabolic response of E. faecalis to an aerobic environment was the upregulation of fatty acid metabolism and benzoate degradation, which was linked to important changes in the bacterial membrane composition as evidenced by changes in membrane fatty acid composition and the reduction of membrane-associated demethylmenaquinone. These key metabolic pathways associated with the response of E. faecalis to oxygen may represent potential new targets to increase the susceptibility of this bacterium to bactericidal drugs. | por |
dc.description.sponsorship | This work was funded by the HRC (Health and Research Council of New Zealand) and the FCT (Portuguese Foundation for Science and Technology), with grant reference SFRH/BD/47016/2008. | por |
dc.language.iso | eng | por |
dc.publisher | American Society for Microbiology (ASM) | por |
dc.rights | openAccess | por |
dc.title | Global metabolic response of Enterococcus faecalis to oxygen | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.comments | CEB17621 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 2012 | por |
oaire.citationEndPage | 2022 | por |
oaire.citationIssue | 11 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationTitle | Journal of bacteriology | por |
oaire.citationVolume | 196 | por |
dc.date.updated | 2014-12-08T16:01:38Z | - |
dc.identifier.eissn | 0021-9193 | - |
dc.identifier.doi | 10.1128/JB.01354-13 | por |
dc.identifier.pmid | 24659768 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of bacteriology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_17621_1.pdf | 2,01 MB | Adobe PDF | Ver/Abrir |