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https://hdl.handle.net/1822/31633
Título: | Candida albicans promotes invasion and colonisation of Candida glabrata in a reconstituted human vaginal epithelium |
Autor(es): | Alves, Carlos Wei, Xiao-Qing Silva, Sónia Carina Azeredo, Joana Henriques, Mariana Williams, David |
Palavras-chave: | RHVE Candidosis Co-infection Candida albicans Candida glabrata Yeast PNA FISH |
Data: | 2014 |
Editora: | W. B. Saunders Co., Ltd. |
Revista: | American Journal of Infection Control |
Citação: | Alves, C. T.; Wei, Xiao-Qing; Silva, Sónia Carina; Azeredo, Joana; Henriques, Mariana; Williams, David W., Candida albicans promotes invasion and colonisation of Candida glabrata in a reconstituted human vaginal epithelium. Journal of Infection, 69(4), 396-407, 2014 |
Resumo(s): | The principal aim of this study was to investigate the in vitro co-infection of a reconstituted human vaginal epithelium (RHVE) by Candida albicans and Candida glabrata. Methods The ability of both species to invade and colonise the RHVE was examined using species-specific peptide nucleic acid (PNA) probe hybridisation, confocal laser scanning microscopy (CLSM) and a novel qRT-PCR protocol for Candida quantification in the tissues. RHVE damage was evaluated by measuring lactate dehydrogenase (LDH) activity. Candida virulence gene expression (HWP1, ALS, EPA, PLB, PLD and SAP) was evaluated by quantitative RT-PCR. Results The results showed that whilst both species induced damage to the RHVE, this was notably less with C. glabrata. Interestingly, there was a significant increase in C. glabrata RHVE colonisation and invasiveness when it was added to the tissue with C. albicans. The extent of RHVE damage caused by the two species appeared to be primarily dependent on the process of invasion. Of the virulence genes assayed, HWP1, PLD1 and ALS3 were deemed to be most associated with pathogenicity in the model. Conclusions For the first time, we have demonstrated that the RHVE model coupled with specific tools of analysis, allows assessment of Candida colonisation and invasion in single and co-infection. Using this model we have demonstrated that C. albicans enhanced C. glabrata colonisation, invasion and tissue damage, which was also evidenced by the expression of virulence genes. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/31633 |
DOI: | 10.1016/j.jinf.2014.06.002 |
ISSN: | 0163-4453 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_17863_1.pdf | 1,21 MB | Adobe PDF | Ver/Abrir |