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https://hdl.handle.net/1822/30520
Título: | Antimycobacterial activity of selected medicinal plants extracts from Cameroon |
Autor(es): | Trigo, Gabriela Neves, Margarida Correia Roque, Susana Donfack, Valerie Flore Donkeng Fokou, Patrick Valere Tsouh Tchokouaha, Lauve Rachel Yamthe Tsabang, Nole Zollo, Paul Henri Amvam Boyom, Fabrice Fekam |
Palavras-chave: | Mycobacterium Tuberculosis Buruli ulcer Antimycobacterial activity Cytotoxicity Annickia chlorantha Ethnobotanical survey |
Data: | 2014 |
Editora: | International Formulae Group (IFG) |
Revista: | International Journal Biological and Chemical Sciences |
Resumo(s): | New drugs are highly needed to control mycobacterial infections. This study aimed at screening ethnobotanically selected plants extracted using organic solvents for their antimycobacterial activity. In vitro assays were performed on Mycobacterium smegmatis, Mycobacterium avium, Mycobacterium bovis Bacille Calmette Guerin (BCG), Mycobacterium tuberculosis and Mycobacterium ulcerans using the Resazurin Microtiter Assay. Cytotoxicity was assessed on Human lung fibroblast cells (MRC5) and bone marrow-derived macrophages (BMDM) using the MTS tetrazolium assay. The most promising extract from Annickia chlorantha stem bark (ACsbI) was tested for intracellular antimycobacterial activity against M. smegmatis using infected BMDM. Sixty crude extracts, 19 fractions, and 2 purified compounds were obtained from 19 Cameroonian medicinal plants. Results showed that crude extracts mainly inhibited BCG, while interface fractions from A. chlorantha stem bark (ACsbI) and stem (ACstI) displayed the strongest activity against M. ulcerans, with Minimal Inhibitory Concentrations (MIC) of 1.95 and 7.81 µg/ml respectively. Two compounds purified from Sorindeia juglandifolia fruits (SJfr 3.6 and SJfr 4.5) showed activity against BCG and M. ulcerans at 3.9 µg/ml and 62.5 µg/ml respectively. Finally, ACsbI showed no toxicity against MRC5 cells and BMDM and inhibited the growth of intracellular M. smegmatis. The results achieved in this investigation support the traditional to use of these plants and the need to investigate them in deeper details to be able to find alternatives for the existing antimycobacterial drugs. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/30520 |
ISSN: | 1991-8631 1997-342X |
Versão da editora: | http://www.ajol.info/index.php/ijbcs/article/view/104072 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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donkeng donfack vf_int j biol chem sci 2014.pdf | 218,93 kB | Adobe PDF | Ver/Abrir |