Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/27512
Título: | Glycol chitosan-based nanogel as a potential targetable carrier for siRNA |
Autor(es): | Pereira, Paula Morgado, Daniela Crepet, Agnès David, Laurent Gama, F. M. |
Palavras-chave: | Cellular uptake and siRNA delivery Cytotoxicity Glycol chitosan nanogel |
Data: | 2013 |
Editora: | Wiley |
Revista: | Macromolecular Bioscience |
Resumo(s): | A self-assembled glycol chitosan nanogel (GC) is synthesized by chemically grafting hydrophobic chains onto a polysaccharide, which is comprehensively characterized. The obtained macromolecular micelle is decorated with folate-conjugated poly(ethylene glycol) (PEG) (GCFA). An average size distribution of 250 and 200 nm is observed, respectively for the GC and GCFA nanogels. Differential cell localization is observed on incubating the materials with HeLa cells. Whereas the GC nanogel is detected on the cell surface, GCFA is localized in the cytoplasm. The cell viability is not compromised by the nanogels. Interestingly, GC nanogel is poorly internalized by bone marrow derived macrophages (BMDMs), and GCFA is not phagocytosed. Given its ability to complex siRNA, the targetable GC nanogel can be a promising vehicle for siRNA delivery. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/27512 |
DOI: | 10.1002/mabi.201300123 |
ISSN: | 1616-5187 |
e-ISSN: | 1616-5195 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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mabi201300123.pdf | 836,37 kB | Adobe PDF | Ver/Abrir |