Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/26707
Título: | A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths |
Autor(es): | Correia, Tiago R. Antunes, Bernardo P. Castilho, Pedro H. Nunes, José C. Amorim, M. T. Pessoa de Escobar, I. C. Correia, I. J. Morão, A. |
Palavras-chave: | Microfiltration Electrospinning Bi-layer membrane Lysate Plasmid DNA DNA Plasmid |
Data: | 2013 |
Editora: | Elsevier 1 |
Revista: | Separation and Purification Technology |
Resumo(s): | The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/26707 |
DOI: | 10.1016/j.seppur.2013.03.049 |
ISSN: | 1383-5866 |
Versão da editora: | http://dx.doi.org/10.1016/j.seppur.2013.03.049 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
1-s2.0-S1383586613001962-main.pdf | 1,61 MB | Adobe PDF | Ver/Abrir |