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https://hdl.handle.net/1822/2565
Título: | Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects |
Autor(es): | Rodrigues, A. C. Wuertz, Stefan Brito, A. G. Melo, L. F. |
Palavras-chave: | Fluorene Phenanthrene Biodegradation CLSM GFP |
Data: | 2005 |
Editora: | John Wiley and Sons |
Revista: | Biotechnology and Bioengineering |
Citação: | "Biotechnology and bioengineering". ISSN 0006-3592. 90:3 (2005) 281-289. |
Resumo(s): | Pseudomonas putida ATCC 17514 was used as a model strain to investigate the characteristics of bacterial growth in the presence of solid fluorene and phenanthrene. Despite the lower water-solubility of phenanthrene, P. putida degraded this polycyclic aromatic hydrocarbon (PAH) at a maximum observed rate of 1.4 ± 0.1 mg Lˉ¹ hˉ¹, higher than the apparent degradation rate of fluorene, 0.8 ± 0.07 mg Lˉ¹ hˉ¹. The role of physiological processes on the biodegradation of these PAHs was analyzed and two different uptake strategies were identified. Zeta potential measurements revealed that phenanthrene-grown cells were slightly more negatively charged (–57.5 ± 4.7 mV) than fluorene-grown cells (–51.6 ± 4.9 mV), but much more negatively charged than glucose-grown cells (–26.8 ± 3.3 mV), suggesting that the PAH substrate induced modifications on the physical properties of bacterial surfaces. Furthermore, protein-to-exopolysaccharide ratios detected during bacterial growth on phenanthrene were typical of biofilms developed under physicochemical stress conditions, caused by the presence of sparingly water-soluble chemicals as the sole carbon and energy source for growth, the maximum value for TP/EPS during growth on phenanthrene (1.9) being lower than the one obtained with fluorene (5.5). Finally, confocal laser microscopy observations using a gfp-labeled derivative strain revealed that, in the presence of phenanthrene, P. putida::gfp cells formed a biofilm on accessible crystal surfaces, whereas in the presence of fluorene the strain grew randomly between the crystal clusters. The results showed that P. putida was able to overcome the lower aqueous solubility of phenanthrene by adhering to the solid PAH throughout the production of extracellular polymeric substances, thus promoting the availability and uptake of such a hydrophobic compound. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/2565 |
DOI: | 10.1002/bit.20377 |
ISSN: | 0006-3592 |
Versão da editora: | http://eu.wiley.com/WileyCDA/ http://www3.interscience.wiley.com/cgi-bin/fulltext/110435733/PDFSTART |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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BB2005-Rodrigues[1].pdf | 830,49 kB | Adobe PDF | Ver/Abrir |