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https://hdl.handle.net/1822/22722
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Campo DC | Valor | Idioma |
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dc.contributor.author | Benelmekki, M. | - |
dc.contributor.author | Xuriguera, E. | - |
dc.contributor.author | Caparrós Vásquez, Cristina Maria | - |
dc.contributor.author | Rodríguez-Carmona, E. | - |
dc.contributor.author | Mendoza, R. | - |
dc.contributor.author | Corchero, J. L. | - |
dc.contributor.author | Lanceros-Méndez, S. | - |
dc.contributor.author | Martínez, Ll. | - |
dc.date.accessioned | 2013-01-18T16:30:01Z | - |
dc.date.available | 2013-01-18T16:30:01Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0021-9797 | por |
dc.identifier.uri | https://hdl.handle.net/1822/22722 | - |
dc.description.abstract | The complete elimination of enzymes from the reaction mixture and the possibility of its recycling for several rounds results in great benefits, allowing the reduction of the enzyme consumption and their usability in continuous processes. In this work it is evaluated the capture of a H6-Tagged GFP (GFP-H6) protein on porous magnetic spheres using the Co2+ and Ni2+ affinity adsorption as a cost effective and up scaled alternative way for the immobilization of his-tagged protein. For this propose, Porous Magnetic Silica (PMS) spheres were synthesised by one step hydrothermal assisted modified-Stöber method. The obtained spheres show a homogeneous size distribution of 400 nm diameter. The -Fe2O3 nanoparticles are homogeneously distributed in the silica matrix. The obtained PMS spheres have a saturation magnetization of about 10 emu/g and a a total separation time of 16 min at 60 T/m was measured by magnetophoresis. The obtained PMS spheres were successfully and homogeneously decorated with Co2+ and Ni2+ and then evaluated for the capture of a GFP-H6 protein. The results were compared with the performance of the commercial beads Dynabeads® His-Tag Isolation & Pulldown. | por |
dc.description.sponsorship | This work is funded by FEDER funds through the "programa Operacional Factores de Competitividade - COMPETE" and by national funds by FCT- Fundacao para a Ciencia e a Tecnologia, project references PEst-C/FIS/U10607/2011, PTDC/CTM/69316/ 2006, and NANO/NMed-SD/0156/2007. The authors also thank support from the COST Action MP1003, 2010 'European Scientific Network for Artificial Muscles'. M.B. thanks the FCT for support under the "Compromisso corn a Ciencia" program. We would like to thank the Protein Production Platform (CIBER-BBN) for helpful technical assistance and for protein production and purification services (http://bbn.ciber-bbn.es/programas/plataformas/equipamiento). The authors appreciate the financial support through the grant IPT-010000-2010-6 (MICINN, INNPACTO subprogram). We also acknowledge the support of the CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), an initiative funded by the VI National R&D&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | restrictedAccess | por |
dc.subject | Magnetophoresis | por |
dc.subject | Magnetic particles | por |
dc.subject | His-Tag proteins | por |
dc.subject | Metal affinity | por |
dc.subject | Surfactant | por |
dc.title | Design and characterization of Ni2+ and Co2+ decorated Porous Magnetic Silica spheres synthesized by hydrothermal-assisted modified-Stober method for His-tagged proteins separation | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/ | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 156 | por |
oaire.citationEndPage | 162 | por |
oaire.citationIssue | 1 | por |
oaire.citationTitle | Journal of Colloid and Interface Science | por |
oaire.citationVolume | 365 | por |
dc.identifier.doi | 10.1016/j.jcis.2011.09.051 | por |
dc.identifier.pmid | 21996010 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Colloid and Interface Science | por |
Aparece nas coleções: | CDF - FCD - Artigos/Papers (with refereeing) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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JCIS-2012.pdf Acesso restrito! | 854,71 kB | Adobe PDF | Ver/Abrir |