Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/22663
Título: | Direct enzymatic esterification of cotton and Avicel with wild-type and engineered cutinases |
Autor(es): | Matamá, Maria Teresa Casal, Margarida Paulo, Artur Cavaco |
Palavras-chave: | Cellulose ester Reverse esterase Transesterification Carbohydrate-binding module Caprylic acid |
Data: | 2013 |
Editora: | Springer |
Revista: | Cellulose |
Resumo(s): | In this work, the surface of cellulose, either Avicel or cotton fabric, was modified using cutinases without any previous treatment to swell or to solubilise the polymer. Aiming further improvement of cutinase ester synthase activity on cellulose, an engineered cutinase was investigated. Wild-type cutinase from Fusarium solani and its fusion with the carbohydrate-binding module N1 from Cellulomonas fimi were able to esterify the hydroxyl groups of cellulose with distinct efficiencies depending on the acid substrate/solvent system used, as shown by titration and by ATR-FTIR. The carbonyl stretching peak area increased significantly after enzymatic treatment during 72 h at 30 °C. Cutinase treatment resulted in relative increases of 31 and 9 % when octanoic acid and vegetable oil were used as substrates, respectively. Cutinase-N1 treatment resulted in relative increases of 11 and 29 % in the peak area when octanoic acid and vegetable oil were used as substrates, respectively. The production and application of cutinase fused with the domain N1 as a cellulose ester synthase, here reported for the first time, is therefore an interesting strategy to pursuit. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/22663 |
DOI: | 10.1007/s10570-012-9827-9 |
ISSN: | 1572-882X 0969-0239 |
Versão da editora: | http://dx.doi.org/10.1007/s10570-012-9827-9 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2012 Direct enzymatic esterification of cotton and Avicel with wild-type and engineered cutinases.pdf | Direct enzymatic esterification of cotton and Avicel with wild-type and engineered cutinases | 152,4 kB | Adobe PDF | Ver/Abrir |