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TitleBamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility
Author(s)Fu, Jiajia
Nyanhongo, Gibson S.
Silva, Carla Manuela Pereira Marinho da
Cardinale, Massimiliano
Prasetyo, E. N.
Yu, Chongwen
Paulo, Artur Cavaco
Gübitz, Georg M.
Issue date2012
PublisherInforma Healthcare
JournalBiocatalysis and Biotransformation
Abstract(s)A biological approach for degumming bamboo substrates has been assessed. The ability of various commercially available enzymes, including cellulase, xylanase, pectinase and mannanase, to hydrolyze bamboo powders was investigated. In addition, a commercial cellulase preparation was applied onto bamboo fibre bundles obtained by natural retting. It was found that almost all enzymes applied can use bamboo material as a substrate. Mild autoclave pre-treatment can enhance reducing sugar yield from different enzyme treatments. A most pronounced effect was observed with cellulase treatment in which the hydrolysis degree increased 1.7 fold as measured by reducing sugars for autoclave pre-treated bamboo powders versus non-treated powders after only a short period of incubation. The combined treatment of hemicellulase preparations showed no effect on the hydrolysis of bamboo substrates. The effect of autoclave pre-treatment on cellulase-treated samples was confirmed by the increase of sugar yield, protein absorption as well as by the enhancement of surface modification and enzyme penetration observed by CLSM (confocal laser scanning microscopy). This work establishes a base for future studies to develop enzymatic hydrolysis of bamboo materials, making them suitable for textile processing.
Publisher version
AccessOpen access
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

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