Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/1772

TítuloConstruction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase
Autor(es)Domingues, Lucília
Onnela, M.
Teixeira, J. A.
Lima, Nelson
Penttilä, M.
Data2000
EditoraSpringer Verlag
RevistaApplied Microbiology and Biotechnology
Citação“Applied microbiology and biotechnology”. ISSN 0175-7598. 54 (2000) 97-103.
Resumo(s)One way of improving heterologous protein production is to use high cell density systems, one of the most attractive being the flocculating yeast production system. Also, lactose is available in large amounts as a waste product from cheese production processes. The construction of flocculent and non-flocculent brewer's yeast strains secreting β-galactosidase and growing on lactose is presented. A plasmid was constructed coding for an extracellular β-galactosidase of Aspergillus niger and having, as selective marker, the yeast CUP1 gene conferring resistance to copper. This selective marker allows for the transformation of wild-type yeasts. This work represents an important step towards the study of heterologous protein secretion by flocculent cells.
TipoArtigo
URIhttps://hdl.handle.net/1822/1772
DOI10.1007/s002530000358
ISSN0175-7598
1432-0614
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
DCILM - Artigos (Papers)

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
2000-11[1].pdf144,5 kBAdobe PDFVer/Abrir

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID