Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1772
Título: | Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase |
Autor(es): | Domingues, Lucília Onnela, M. Teixeira, J. A. Lima, Nelson Penttilä, M. |
Data: | 2000 |
Editora: | Springer Verlag |
Revista: | Applied Microbiology and Biotechnology |
Citação: | “Applied microbiology and biotechnology”. ISSN 0175-7598. 54 (2000) 97-103. |
Resumo(s): | One way of improving heterologous protein production is to use high cell density systems, one of the most attractive being the flocculating yeast production system. Also, lactose is available in large amounts as a waste product from cheese production processes. The construction of flocculent and non-flocculent brewer's yeast strains secreting β-galactosidase and growing on lactose is presented. A plasmid was constructed coding for an extracellular β-galactosidase of Aspergillus niger and having, as selective marker, the yeast CUP1 gene conferring resistance to copper. This selective marker allows for the transformation of wild-type yeasts. This work represents an important step towards the study of heterologous protein secretion by flocculent cells. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/1772 |
DOI: | 10.1007/s002530000358 |
ISSN: | 0175-7598 1432-0614 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DCILM - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2000-11[1].pdf | 144,5 kB | Adobe PDF | Ver/Abrir |