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https://hdl.handle.net/1822/11349
Título: | Solute partitioning in polymer–salt ATPS: the collander equation |
Autor(es): | Silvério, Sara C. Rodríguez, Oscar Teixeira, J. A. Macedo, Eugénia |
Palavras-chave: | Aqueous two-phase system Polymer–salt Collander equation Proteins |
Data: | 2010 |
Editora: | Elsevier 1 |
Revista: | Fluid Phase Equilibria |
Citação: | "Fluid Phase Equilibria". ISSN 0378-3812. 296:2 (2010) 173-177. |
Resumo(s): | The partition coefficients for several solutes (five nitrophenylated-monosaccharides and four proteins) were experimentally determined, at 23 ◦C, in three different tie-lines of two polymer–salt aqueous twophase systems (ATPS): UCON-K2HPO4 and UCON-NaH2PO4. These partition coefficients together with others obtained from the literature for five dinitrophenylated-amino acids were used to investigate the suitability of the Collander equation to correlate partition coefficients in polymer–salt ATPS. This equation was first proposed to describe the linear correlation between partition coefficients of solutes in different water–organic solvent systems. More recently, it was proved that partition coefficients for several biomolecules in polymer–polymer ATPS can also be correlated with this equation. In this work, several correlations were tested: partition coefficients obtained for different tie-lines within the same system and also partition coefficients obtained from different systems. In both cases, a linear relation was observed, despite a less satisfactory correlation was found when different ATPS were compared. Overall, it was demonstrated that the Collander equation can be used to satisfactorily correlate solute partitioning in the studied polymer–salt ATPS. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/11349 |
DOI: | 10.1016/j.fluid.2010.04.009 |
ISSN: | 0378-3812 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Silvério_Fluid Phase Equilibria.pdf | 311,73 kB | Adobe PDF | Ver/Abrir |