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https://hdl.handle.net/1822/11223
Título: | Characterisation of Candida parapsilosis infection of an in vitro reconstituted human oral epithelium |
Autor(es): | Silva, Sónia Carina Henriques, Mariana Oliveira, Rosário Azeredo, Joana Malic, Sladjana Hooper, Samuel J. Williams, David |
Palavras-chave: | Candida Candidosis Candida parapsilosis Epithelium Oral |
Data: | Dez-2009 |
Editora: | Wiley |
Revista: | European Journal of Oral Sciences |
Citação: | "European Journal of Oral Sciences." ISSN 0909-8836. 117:6 (Dez. 2009) 669–675. |
Resumo(s): | Oral candidosis is a common problem in immunocompromised patients, and whilst Candida albicans is regarded as the principal cause of infection, other non-Candida albicans Candida (NCAC) species are increasingly being recognized as human pathogens. Relatively little is known about the virulence factors associated with NCAC species, and the aim of this study was to use a reconstituted human oral epithelium (RHOE) to examine epithelial infection with Candida parapsilosis. Strains originating from the oral and vaginal mucosa and from the urinary tract were all shown to colonize RHOE in a strain-dependent manner. Strain differences were found in the colonizing morphology and in the extent of invasion of the RHOE. Low invasion of RHOE was detected for strains after 12 h, whereas extensive tissue damage was evident after 24 h when assessed using histological examination and lactate dehydrogenase activity determination. Tissue damage was reduced in the presence of pepstatin A, although C. parapsilosis invasion of the tissue was not inhibited. Realtime polymerase chain reaction of secreted aspartyl proteinase (SAP) genes (SAPP1– 3) showed that expression was strain dependent, with an increased expression generally occurring for Candida infecting RHOE compared with planktonic equivalents. In summary, C. parapsilosis was not highly invasive of RHOE but did induce significant tissue damage, which could relate to specific SAP gene expression. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/11223 |
DOI: | 10.1111/j.1600-0722.2009.00677.x |
ISSN: | 0909-8836 1600-0722 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Silva_European Journal of Oral Sciences.pdf Acesso restrito! | 628,52 kB | Adobe PDF | Ver/Abrir |