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TitleBiomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions
Author(s)Bai, Luge
Hao, Zhiyan
Wang, Sen
Zhou, Jiajia
Yao, Siqi
Pei, Na
Zhu, Hui
Zhang, Kun
Reis, R. L.
Oliveira, Joaquim M.
He, Jiankang
Li, Dichen
Mao, Xinggang
Wang, Ling
Glioma model
In vitro 3D model
3D bioprinting
Glioma cells
Issue dateMar-2023
PublisherAccScience Publishing
JournalInternational Journal of Bioprinting
CitationBai L., Hao Z., Wang S., Zhou J., Yao S., Pei N., Zhu H., Zhang K., Reis R. L., Oliveira J. M., He J., Li D., Mao X., Wang L. Biomimetic three-dimensional glioma model printed in vitro for the studies of glioma cells and neurons interactions, International Journal of Bioprinting, Vol. 9, Issue 4, doi:10.18063/ijb.715, 2023
Abstract(s)The interactions between glioma cells and neurons are important for glioma progression but are rarely mimicked and recapitulated in in vitro three-dimensional (3D) models, which may affect the success rate of relevant drug research and development. In this study, an in vitro bioprinted 3D glioma model consisting of an outer hemispherical shell with neurons and an inner hemisphere with glioma cells is proposed to simulate the natural glioma. This model was produced by extrusion-based 3D bioprinting technology. The cells survival rate, morphology, and intercellular Ca2+ concentration studies were carried out up to 5 days of culturing. It was found that neurons could promote the proliferation of glioma cells around them, associate the morphological changes of glioma cells to be neuron-like, and increase the expression of intracellular Ca2+ of glioma cells. Conversely, the presence of glioma cells could maintain the neuronal survival rate and promote the neurite outgrowth. The results indicated that glioma cells and neurons facilitated each other implying a symbiotic pattern established between two types of cells during the early stage of glioma development, which were seldom found in the present artificial glioma models. The proposed bioprinted glioma model can mimic the natural microenvironment of glioma tissue, provide an in-depth understanding of cellâ cell interactions, and enable pathological and pharmacological studies of glioma.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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