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TitleHeadspace - SPME as a screening system for yeast metabolic compounds: a case study
Author(s)Martins, Margarida Isabel Barros Coelho
Henriques, Mariana
Azeredo, Joana
Oliveira, Rosário
KeywordsHS - SPME
Candida species
Isoamyl alcohol
Issue dateSep-2008
PublisherUniversidade do Minho. Departamento de Engenharia Biológica (DEB)
CitationMARTINS, Margarida Rangel [et al.] - Headspace - SPME as a screening system for yeast metabolic compounds : a case study. In FERREIRA, E. C.; MOTA, M., ed. lit. – “Chempor 2008 : proceedings of the International Chemical and Biological Engineering Conference, 10, Braga, 2008” [CD-ROM]. Braga : Departamento de Engenharia Biológica da Universidade do Minho, 2008. ISBN 978-972-97810-3-2.
Abstract(s)Aim: This study aimed to identify and quantify the fusel alcohols secreted by the opportunistic yeasts Candida albicans and Candida dubliniensis to further get insights into the amino acid catabolism of those two organisms. Methods and Results: Fusel alcohols were screened in cell - free supernatants of yeasts cultivated in RPMI medium. For simultaneous identification and quantification of these alcohols, a Headspace - Solid Phase Micro Extraction (HS - SPME) coupled with gas chromatography - mass spectrometry (GC - MS) methodology, previously developed to analyse the volatile composition of fruits, was followed (Coelho et al. 2006). Results obtained show that both C. albicans and C. dubliniensis produced isoamyl alcohol and 2- phenylethanol, end – products of the catabolism of the amino acids leucine and phenylalanine, respectively. Significance and Impact of the study: The HS – SPME used herein allows the simultaneous identification and quantification of compounds, requires small sample volume, has high sensitivity and reproducibility, is simple and rapid and is solvent free. This revealed to be a powerful tool for the screening system of yeast metabolic compounds.
TypeConference paper
AccessOpen access
Appears in Collections:CEB - Artigos em Livros de Atas / Papers in Proceedings

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