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https://hdl.handle.net/1822/8355
Título: | Characterization of biofilm formation on a humic material |
Autor(es): | Rodrigues, A. L. Brito, A. G. Janknecht, P. Silva, J. Machado, A. V. Nogueira, R. |
Palavras-chave: | Humic material Biofilm Flowcell Community composition FTIR |
Data: | Nov-2008 |
Editora: | Springer |
Revista: | Journal of Industrial Microbiology and Biotechnology |
Citação: | “Journal of Industrial Microbiology and Biotechnology”. ISSN 1367-5435. 35:11 (2008) 1269–1276. |
Resumo(s): | Biofilms are major sites of carbon cycling in streams. Therefore, it is crucial to improve knowledge about biofilms’ structure and microbial composition to understand their contribution in the self-purification of surface water. The present work intends to study biofilm formation in the presence of humic substances (HSs) as a carbon source. Two biofilm flowcells were operated in parallel; one with synthetic stream water, displaying a background carbon concentration of 1.26 ± 0.84 mg L−1, the other with added HSs and an overall carbon concentration of 9.68 ± 1.00 mg L−1. From the biofilms’ results of culturable and total countable cells, it can be concluded that the presence of HSs did not significantly enhance the biofilm cell density. However, the biofilm formed in the presence of HSs presented slightly higher values of volatile suspended solids (VSS) and protein. One possible explanation for this result is that HSs adsorbed to the polymeric matrix of the biofilm and were included in the quantification of VSS and protein. The microbial composition of the biofilm with addition of HSs was characterized by the presence of bacteria belonging to beta-Proteobacteria, Cupriavidus metallidurans and several species of the genus Ralstonia were identified, and gamma-Proteobacteria, represented by Escherichia coli. In the biofilm formed without HSs addition beta-Proteobacteria, represented by the species Variovorax paradoxus, and bacteria belonging to the group Bacteroidetes were detected. In conclusion, the presence of HSs did not significantly enhance biofilm cell density but influenced the bacterial diversity in the biofilm. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/8355 |
DOI: | 10.1007/s10295-008-0424-8 |
ISSN: | 1367-5435 1476-5535 |
Versão da editora: | http://www.springerlink.com/content/47j220118p802581/?p=4d5c00415fe741fd98cb4f08890a0ce2&pi=10 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series IPC - Artigos em revistas científicas internacionais com arbitragem |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Rodrigues_JIMB[1].pdf | 389,46 kB | Adobe PDF | Ver/Abrir |