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TitleHow useful is the assessment of lymphatic vascular density in oral carcinoma prognosis?
Author(s)Longatto Filho, Adhemar
Oliveira, Tiago Gil
Pinheiro, Céline
Carvalho, Marcos Brasilino
Curioni, Otávio Alberto
Mercante, Ana Maria da Cunha
Schmitt, Fernando C.
Gattás, Gilka J. F.
Issue date11-Dec-2007
PublisherBioMed Central (BMC)
JournalWorld Journal of Surgical Oncology
Citation"World Journal of Surgical Oncology". ISSN 1477-7819. 5:140 (Dez. 2007).
Abstract(s)Background: Lymphatic vessels are major routes for metastasis in head and neck squamous cell carcinoma (HNSCC), but lymphatic endothelial cells (LECs) are difficult to recognize in tumor histological sections. D2-40 stains podoplanin, a molecule expressed in LECs, however, the potential prognostic usefulness of this molecule is not completely understood in HNSCC. We aimed to investigate the value of assessing peritumoral and intratumoral lymphatic vessel density (LVD) as prognostic marker for HNSCC. Methods: Thirty-one cases of HNSCC were stained for D2-40 and CD31. LVD and blood vessel density (BVD) were assessed by counting positive reactions in 10 hotspot areas at ×200 magnification. Results: D2-40 was specific for lymphatic vessels and did not stain blood vascular endothelial cells. LECs showed more tortuous and disorganized structure in intratumoral lymphatic vessels than in peritumoral ones. No statistical differences were observed between peritumoral-LVD and intratumoral-LVD or between peritumoral-BVD and intratumoral-BVD. Tumor D2-40 staining was positively associated with lymphatic vessel invasion (p = 0.011). Conclusion: LVD is a powerful marker for HNSCC prognosis. We found significant differences in peritumoral and intratumoral D2-40 immunoreactivity, which could have important implications in future therapeutic strategies and outcome evaluation.
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AccessOpen access
Appears in Collections:ICVS - Artigos em revistas internacionais / Papers in international journals

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