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TitleStudy of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications
Author(s)Alves, A. L.
Costa-Gouveia, J.
Vieira de Castro, J.
Sotelo, C. G.
Vázquez, J. A.
Pérez Martín, R. I.
Torrado, E.
Neves, N. M.
Reis, R. L.
Castro, A. G.
Silva, Tiago H.
KeywordsAnti-inflammatory macrophages
M2-like macrophages
marine biomaterials
type I collagen
Issue dateJan-2022
JournalActa Biomaterialia
CitationAlves A. L., Costa-Gouveia J., Vieira de Castro J., Sotelo C. G., Vázquez J. A., Pérez Martín R. I., Torrado E., Neves N. M., Reis R. L., Castro A. G., Silva T. H. Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications, Acta Biomaterialia, Vol. 141, pp. 123-131, doi:10.1016/j.actbio.2022.01.009, 2022
Abstract(s)The host immunologic response to a specific material is a critical aspect when considering it for clinical implementation. Collagen and gelatin extracted from marine sources have been proposed as biomaterials for tissue engineering applications, but there is a lack of information in the literature about their immunogenicity. In this work, we evaluated the immune response to collagen and/or gelatin from blue shark and codfish, previously extracted and characterized. After endotoxin evaluation, bone marrow-derived macrophages were exposed to the materials and a panel of pro- and anti-inflammatory cytokines were evaluated both for protein quantification and gene expression. Then, the impact of those materials in the host was evaluated through peritoneal injection in C57BL/6 mice. The results suggested shark collagen as the less immunogenic material, inducing low expression of pro-inflammatory cytokines as well as inducible nitric oxide synthase (encoded by Nos2) and high expression of Arginase 1 (encoded by Arg1). Although shark gelatin appeared to be the material with higher pro-inflammatory expression, it also presents a high expression of IL-10 (anti-inflammatory cytokine) and Arginase (both markers for M2-like macrophages). When injected in the peritoneal cavity of mice, our materials demonstrated a transient recruitment of neutrophil, being almost non-existent after 24 hours of injection. Based on these findings, the studied collagenous materials can be considered interesting biomaterial candidates for regenerative medicine as they may induce an activation of the M2-like macrophage population, which is involved in suppressing the inflammatory processes promoting tissue remodeling.
DescriptionSupplementary material associated with this article can be found, in the online version, at doi: 10.1016/j.actbio.2022.01.009 .
Publisher version
AccessEmbargoed access (2 Years)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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