Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/73411
Título: | Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum |
Autor(es): | Oliveira, Ana Isabel Pinho, Cláudia Fonte, Pedro Sarmento, Bruno Dias, Alberto Carlos Pires |
Palavras-chave: | Hep G2 Cells Humans Hypericum perforatum Antioxidants Drug Carriers Nanoparticles Neuroprotective Agents Polyesters Quercetin Biapigenin Hepatoprotective In vitro release Anti-oxidant Anti-oxidant |
Data: | 2018 |
Editora: | Elsevier |
Revista: | International Journal of Biological Macromolecules |
Citação: | Oliveira, A. I., Pinho, C., Fonte, P., Sarmento, B., & Dias, A. C. P. (2018). Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum. International Journal of Biological Macromolecules, 110, 185-196. doi: https://doi.org/10.1016/j.ijbiomac.2017.10.103 |
Resumo(s): | Quercetin and biapigenin are antioxidant and neuroprotective compounds present in Hypericum perforatum, with potential application in neurodegenerative diseases. Quercetin has shown to have excellent antioxidant activities. Biapigenin possesses a distinctive mechanism of action, preventing the onset of calcium deregulation and mitochondrial dysfunction. The main aim of this study was to establish a new delivery system encapsulating H. perforatum neuroprotective fraction, isolated from it,containing quercetin and biapigenin into poly(Ɛ-caprolactone) (PCL) nanoparticles. It also aimed to study its hepatoprotective potential. Different formulation parameters were optimized, resulting in a stable formulation of polymer:compounds ratio 1:0.1 with a mean particle size of 185nm, zeta potential around -20mV and association efficiency close to 100%. Compounds in vitro release, under physiological conditions, showed an initial burst followed by a sustained release. The antioxidant functional properties of these compounds were not altered by encapsulation. PCL-loaded nanoparticles protected HepG2 cells from intrinsic compound toxicity at high concentrations. Depending on the incubation regimen, quercetin-biapigenin PCL-loaded nanoparticles or free compounds were more effective in protecting HepG2 cells against tert-butylhydroperoxide-induced toxicity. This is the first report of the encapsulation of a quercetin-biapigenin mixture in a polymeric matrix, specifically, in PCL, with synergic anti-oxidant and hepatoprotective effects. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/73411 |
DOI: | 10.1016/j.ijbiomac.2017.10.103 |
ISSN: | 0141-8130 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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(2018) oliveira.pdf | 2,25 MB | Adobe PDF | Ver/Abrir |