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TitleStructure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio
Author(s)Martin-Pastor, M.
Ferreira, A. S.
Moppert, X.
Nunes, C.
Coimbra, M. A.
Reis, R. L.
Guezennec, J.
Novoa-Carballal, Ramon
Hyaluronic acid
NMR spectroscopy
Physicochemical properties
Bacterial mats
Vibrio sp
Issue dateOct-2019
JournalCarbohydrate Polymers
CitationMartin-Pastor M., Ferreira A. S., Moppert X., Nunes C., Coimbra M. A., Reis R. L., Guezennec J., Novoa-Carvallal R. Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio, Carbohydrate Polymers, Vol. 222, pp. 1149992, doi:10.1016/j.carbpol.2019.114999, 2019
Abstract(s)MO245 exopolysaccharide (EPS) was produced in laboratory conditions from Vibrio genus microorganism isolated from bacterial mats found in Moorea Island. Its structure consists of a linear tetrasaccharide repeating unit â 4)-β-D-GlcpA-(1â 4)-α-D-GalpNAc-(1â 3)-β-D-GlcpNAc-(1â 4)-β-D-GlcpA-(1â containing covalently-linked 5% of glucose, galactose, and rhamnose, determined by methylation analyses and NMR spectroscopy. The molecular weight, radius of gyration (Rg) and intrinsic viscosity, [η], determined by gel permeation chromatography with light scattering and viscosity detection, were 513â ±â 4â kDa (PDI, 1.42â ±â 0.01), 6.7â ±â 0.3â dl/g and 56â ±â 0.3â nm respectively. The chelation of the EPS with copper divalent ions leads to the instantaneous formation of gels. The structural similitude proposed, based in an equal ratio of GlcA to N-acetylated sugars and in the same type of glyosidic linkages present in the repeating unit (alternated 1â 3 and 1â 4 linkages), is translated into analogous physicochemical properties: MO245 EPS is a flexible polyelectrolyte, with scaling exponents similar to that described for HA. This similitude opens opportunities in future drug delivery, tissue engineering, and cosmetic applications.
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Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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