Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/58239

TitleSTATs in lung development: distinct early and late expression, growth modulation and signaling dysregulation in congenital diaphragmatic hernia
Author(s)Piairo, Paulina C.
Moura, Rute S.
Baptista, Maria João Ribeiro Leite
Correia-Pinto, Jorge
Silva, Cristina Isabel Nogueira
KeywordsAnimals
Female
Fetal Development
Gene Expression
Hernias, Diaphragmatic, Congenital
Immunohistochemistry
Lung
Phenyl Ethers
Rats
Rats, Sprague-Dawley
STAT Transcription Factors
STAT3 Transcription Factor
STAT6 Transcription Factor
Stilbenes
Suppressor of Cytokine Signaling 3 Protein
Signal transducer and activator of transcription (STAT)
Suppressor of cytokine signaling (SOCS)
Congenital diaphragmatic hernia (CDH)
Lung development
Nitrofen
Issue dateFeb-2018
PublisherKarger Publishers
JournalCellular Physiology and Biochemistry
Abstract(s)Congenital diaphragmatic hernia (CDH) is a life-threatening developmental anomaly, intrinsically combining severe pulmonary hypoplasia and hypertension. During development, signal transducers and activators of transcription (STAT) are utilized to elicit cell growth, differentiation, and survival.Background: Congenital diaphragmatic hernia (CDH) is a life-threatening developmental anomaly, intrinsically combining severe pulmonary hypoplasia and hypertension. During development, signal transducers and activators of transcription (STAT) are utilized to elicit cell growth, differentiation, and survival. Methods: We used the nitrofen-induced CDH rat model. At selected gestational time points, lungs were divided into two experimental groups, i.e., control or CDH. We performed immunohistochemistry and western blotting analysis to investigate the developmental expression profile of the complete family of STATs (STAT1-6), plus specific STATs activation (p-STAT3, p-STAT6) and regulation by SOCS (SOCS3) in normal lungs against those of diseased lungs. The normal fetal lung explants were treated with piceatannol (STAT3 inhibitor) in vitro followed by morphometrical analysis. Results: Molecular profiling of STATs during the lung development revealed distinct early and late expression signatures. Experimental CDH altered the STATs expression, activation, and regulation in the fetal lungs. In particular, STAT3 and STAT6 were persistently over-expressed and early over-activated. Piceatannol treatment dose-dependently stimulated the fetal lung growth. Conclusion: These findings suggest that STATs play an important role during normal fetal lung development and CDH pathogenesis. Moreover, functionally targeting STAT signaling modulates fetal lung growth, which highlights that STAT3 and STAT6 signaling might be promising therapeutic targets in reducing or preventing pulmonary hypoplasia in CDH.
TypeArticle
URIhttp://hdl.handle.net/1822/58239
DOI10.1159/000486218
ISSN1015-8987
e-ISSN1421-9778
Publisher versionhttps://www.karger.com/Article/FullText/486218
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em revistas internacionais / Papers in international journals

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