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https://hdl.handle.net/1822/57899
Título: | Inflammatory response and long-term behavioral assessment after neonatal CO2-pneumothorax: study in a rodent model |
Autor(es): | Miranda, Alice Augusta Macedo Roque, Susana Miranda, Cláudia Sofia Serre Pêgo, José M. Correia-Pinto, Jorge |
Palavras-chave: | Animals Animals, Newborn Carbon Dioxide Cytokines Dentate Gyrus Disease Models, Animal Interleukin-10 Leukocytes Male Pneumothorax, Artificial Rats Rats, Sprague-Dawley Behavior, Animal Neonate Minimally invasive surgery Pneumothorax CO2-insufflation Inflammation CO -insufflation 2 |
Data: | Jul-2018 |
Editora: | Elsevier Inc |
Revista: | Journal of Pediatric Surgery |
Citação: | Miranda, A., Roque, S., Serre-Miranda, C., Pêgo, J. M., & Correia-Pinto, J. (2018). Inflammatory response and long-term behavioral assessment after neonatal CO2-pneumothorax: study in a rodent model. Journal of pediatric surgery, 53(7), 1318-1325 |
Resumo(s): | Background: Carbon-dioxide (CO2)-pneumothorax during minimally invasive surgery induces well-known metabolic changes. However, little is known about its impact on the central nervous system.The aim of this work is to evaluate the acute impact of CO2-pneumothorax over central cytokine response and its long-term effect on animal behavior.Methods: This is an experimental study where neonatal Sprague-Dawley rats are submitted to CO2-pneumothorax. Peripheral and central cytokine response was evaluated 24 h after insufflation, and peripheral immune cell phenotyping was evaluated 24 h and 4 weeks post-insufflation. Progenitor cell survival was evaluated in the hippocampal dentate gyrus, and the behavioral analysis was performed in adulthood to test cognition, anxious-like, and depressive-like behavior.Results: Significantly increased 11-10 levels were observed in the cerebrospinal-fluid (CSF) of animals submitted to CO2-pneurnothorax, while no differences were found in serum. Regarding pro-inflammatory cytokines, no differences were observed in the periphery or centrally. CO2-pneumothorax event did not alter the survival of newborn cells in the hippocampal dentate gyrus, and no impact on long-term behavior was observed.Conclusions: Neonatal animals submitted to CO2-pneumothorax present acutely increased CSF IL-10 levels. The CO2 pneumothorax seems to result in no significant outcome over neurodevelopment as no functional behavioral alterations were observed in adulthood. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/57899 |
DOI: | 10.1016/j.jpedsurg.2017.08.016 |
ISSN: | 0022-3468 |
e-ISSN: | 1531-5037 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0022346817305079 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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miranda2017.pdf | 1,17 MB | Adobe PDF | Ver/Abrir |