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https://hdl.handle.net/1822/56676
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Campo DC | Valor | Idioma |
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dc.contributor.author | Canadas, Raphael Faustino | por |
dc.contributor.author | Ren, Tachen | por |
dc.contributor.author | Marques, A. P. | por |
dc.contributor.author | Oliveira, J. M. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Demirci, Utkan | por |
dc.date.accessioned | 2018-10-30T13:42:49Z | - |
dc.date.available | 2021-01-01T07:00:46Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Canadas R. F., Ren T., Marques A. P., Oliveira J. M., Reis R. L., Demirci U. Biochemical Gradients to Generate 3D Heterotypic-Like Tissues with Isotropic and Anisotropic Architectures, Advanced Functional Materials, Issue 1804148, doi: 10.1002/adfm.201804148, 2018 | por |
dc.identifier.issn | 1616-3028 | por |
dc.identifier.uri | https://hdl.handle.net/1822/56676 | - |
dc.description.abstract | Anisotropic 3D tissue interfaces with functional gradients found in nature are replicated in vitro for drug development and tissue engineering. Even though different fabrication techniques, based on material science engineering and microfluidics, are used to generate such microenvironments, mimicking the native tissue gradient is still a challenge. Here, the fabrication of 3D structures are described with linear/random porosity and gradient distribution of hydroxyapatite microparticles which are combined with a gradient of growth factors generated by a dual chamber for the development of heterotypicâ like tissues. The hydroxyapatite gradient is formed by applying a thermal ramp from the first to the second gel layer, and the porous architecture is controlled through ice templating. A 3D osteochondral (OC) tissue model is developed by codifferentiating fat pad adiposeâ derived stem cells. Osteogenic and chondrogenic markers expression is spatially controlled, as it occurs in the native osteochondral unit. Additionally, a prevasculature is spatially induced by the perfusion of proangiogenic medium in the boneâ like region, as observed in the native subchondral bone. Thus, in this study, precise spatial control is developed over cell/tissue phenotype and formation of prevasculature which opens up possibilities for the study of complex tissues interfaces, with broader applications in drug testing and regenerative medicine. | por |
dc.description.sponsorship | The authors are grateful for the Fundação para a Ciência e a Tecnologia (FCT) distinctions attributed to R. F. Canadas (SFRH/ BD/92565/2013), who was awarded a PhD scholarship and to J.M.O. (IF/00423/2012 and IF/01285/2015). The authors acknowledge that this material and collaboration is based in part upon work supported by Luso-American Development Foundation (FLAD). This work was supported in part by the European Research Council Grant agreement ERC-2012-ADG 20120216-321266 for project ComplexiTE. The authors would like to acknowledge the National Science Foundation under Award No. NSF 1547791 and by the Office of the Assistant Secretary of Defense for Health Affairs under Award No. W81XWH-15-1-0576. Opinions, interpretations, conclusions, and recommendations are those of the author and are not necessarily endorsed by the National Institutes of Health or the Department of Defense. The authors also thank SAR—Soluções de Automação e Robótica for the support with bioreactor development, to João Costa (3B’s Research Group) for the assistance with dynamic mechanical analysis, to I. F. Cengiz (3B’s Research Group) and Filipe Carvalho (3B’s Research Group) for their help with material shipment and micro-CT analysis, and to Alessandro Tocchio (BAMM Lab) for their helpful and inspiring discussions. | por |
dc.language.iso | eng | por |
dc.publisher | Wiley | por |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F92565%2F2013/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00423%2F2012%2FCP0177%2FCT0004/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F01285%2F2015%2FCP1294%2FCT0008/PT | - |
dc.rights | openAccess | por |
dc.subject | 3D | por |
dc.subject | Anisotropic | por |
dc.subject | Bioreact | por |
dc.subject | Bioreactor | por |
dc.subject | Co-culture | por |
dc.subject | Dynamic | por |
dc.subject | Gradient | por |
dc.subject | Model | por |
dc.subject | Osteochondral | por |
dc.subject | anisotropy | por |
dc.subject | biochemical gradients | por |
dc.subject | heterotypic tissues | por |
dc.subject | microparticle gradients | por |
dc.title | Biochemical gradients to generate 3D heterotypic-like tissues with isotropic and anisotropic architectures | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201804148 | por |
dc.comments | http://3bs.uminho.pt/node/19653 | por |
oaire.citationIssue | 1804148 | por |
oaire.citationVolume | 28 | por |
dc.date.updated | 2018-10-30T12:05:12Z | - |
dc.identifier.doi | 10.1002/adfm.201804148 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Advanced Functional Materials | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19653-Canadas_et_al-2018-Advanced_Functional_Materials.pdf | 12,09 MB | Adobe PDF | Ver/Abrir |